中文摘要：

利用具有降解己烯雌酚（diethylstilbestrol,DES）特性的功能微生物来降解DES、有望实现环境中DES的有效去除,然而迄今关于降解DES的功能菌株及其降解特性的报道很少.本研究通过选择性富集培养,从某污水处理站活性污泥中分离获得1株具有DES降解特性的细菌（菌株S）.经形态观察、生理生化试验及16S rDNA序列同源性分析将菌株S鉴定为沙雷氏菌属（Serratia sp.）细菌.菌株S好氧生长,30℃、150 r·min^-1摇床培养7 d后,对无机盐培养基中DES（50 mg·L^-1）的降解率达68.3%.通过摇瓶实验,优化了菌株S生长和降解DES的最适环境条件：温度30℃,底物浓度40～60 mg·L^-1,pH 7.0,接种量5%,盐添加量0 g·L^-1,装液量10 mL（100 mL三角瓶）.

英文摘要：

Utilizing the diethylstilbestrol（DES）-degrading bacteria to biodegrade DES is a most reliable technique for cleanup of DES pollutants from the environment. However,little information is available heretofore on the isolation of DES-degrading bacteria and their DES removal performance in the environment. A novel bacterium capable of degrading DES was isolated from the activated sludge of a wastewater treatment plant. According to its morphology,physiochemical characteristics,and 16 S rDNA sequence analysis,this strain was identified as Serratia sp.. The strain was an aerobic bacterium,and it could degrade 68. 3% of DES（50 mg·L^-1） after culturing for 7 days at 30℃,150 r·min^-1 in shaking flasks. The optimal conditions for DES biodegradation by the obtained strain were 30℃,40-60 mg·L^-1DES,pH 7. 0,5% of inoculation volume,0 g·L^-1of added NaCl,and 10 mL of liquid medium volume in 100 mL flask.