中文摘要：

通过电聚合方法和脉冲沉积技术将普鲁士蓝（PB）与，L-半胱氨酸（L—Cys）修饰在玻碳电极（GCE）表面，制得复合膜化学修饰电极（L—Cys／PB／GCE／CME），利用循环伏安法和计时安培法研究了对苯二酚在L—Cys／PB／GCE／CME上的电化学特征。结果表明，在0．1mol／LPBS（pH7．0）缓冲溶液中，L—Cys／PB／GCE／CME对对苯二酚的电化学氧化具有明显的催化作用，氧化峰电流相对于在裸玻碳电极上增加了5倍。在最佳实验条件下，对苯二酚浓度在0．18～120μmol／L范围内，修饰电极的电流响应与对苯二酚浓度呈线性关系，其相关系数为0．9962，检出限（S／N=3）为0．065μmoX／L。本研究制备的对苯二酚传感器具有较好的重复性、重现性、选择性与稳定性，用于实际水样中对苯二酚的测定，结果满意。

英文摘要：

A glassy carbon electrode （GCE） modified by Prussian blue （PB） and L-Cysteine （L-Cys） composite film was prepared by the method of electro-polymerization and pulse electrodeposition. The electrochemical performance of PB/L-Cys/GCE/CME for detection of hydroquinone was investigated by cyclic vohammetry and chronoamperometry. The results showed that in 0. 1 mol/L PBS （pH 7.0） buffer, PB/L- Cys/GCE/CME exhibited excellent catalytic and enhancement effect on the electrochemical oxidation of hydroquinone, and the oxidation peak current increased ca. 5 times as compared with that at bare GCE. Under the optimal conditions, hydroquinone concentration was linear with peak current in the range of 0.18- 120 μmol/L with a correlation coefficient of 0. 9962, and the detection limit was 0. 065 μmol/L （ S/N= 3 ）. The proposed hydroquinone biosensor has good repeatability, reproducibility, selectivity and stability, and can be applied to determine the hydroquinone content in water sample.