中文摘要：

目的：明确转录因子Sp1和Sp3对食管癌细胞ezrin基因的表达调控作用。方法：将转录因子表达载体CMV-Sp1和CMV-Sp3分别转染食管癌EC109细胞,采用定量RT-PCR和Western blot技术检测过表达转录因子Sp1和Sp3对ezrinmR-NA和蛋白表达的影响;将ezrin基因启动子驱动的报告基因表达载体与内参照质粒pRL-TK和转录因子表达载体共转染EC109细胞,采用双荧光素酶报告基因分析系统检测转录因子Sp1和Sp3对ezrin基因启动子的激活作用以及这种激活作用是否依赖于ezrin基因的Sp1结合位点-75/-69。结果：过表达转录因子Sp1和Sp3显著提高EC109细胞ezrinmRNA和蛋白表达水平以及启动子活性。Sp1和Sp3增强ezrin基因启动子活性的作用位点不同,只有Sp1通过-75/-69位点调控ezrin基因启动子活性。结论：转录因子Sp1和Sp3可调控EC109细胞ezrin基因的表达。

英文摘要：

Objective： To identify the role of transcription factors Spl and Sp3 in the expressional regulation of ezrin in htunan esophageal carcinoma cells.Methods： Esophageal carcinoma EC109 cells were transfected with expressing vectors CMV-Sp1 or CMV-Sp3, and the effect of Sp1 and Sp3 over-expression on ezr/n mRNA and protein expression was determined by real time RT-PCR and Western blot analysis. Furthermore, EC109 cells were cotransfected with the ezr/n promoter-directed luciferase reporter vector and control vector pRL-TK along with transcription factor expression vector. The roles of Spl and Sp3 in ezrin promoter activation and whether this activation occurred through the Spl binding site,-75/-69, were analyzed by dual-luciferase reporter assay system. Results： Over-expression of transcription factors Sp1 and Sp3 significantly increased the expression of ezrin mRNA and protein and the ezrin promoter activity in EC109 cells. Sp1 and Sp3 enhanced the promoter activity through different binding sites and only Spl did that through the -75/-69 site. Conclusion： Spl and Sp3 can regulate ezrin expression in EC109 cells.