中文摘要：

以野扁桃为材料,对影响ISSR-PCR扩增结果的Mg^2＋、dNTPs、Taq酶、模板DNA、引物等因素进行筛选和优化,确立了适合野扁桃ISSR分析的最佳反应体系：25μL PCR反应体系中包含1×Buffer,2.0 mmol.L-1Mg^2＋,160μmol.L-1dNTPs,模板DNA30 ng,Taq聚合酶1 U,引物15 pmol。

英文摘要：

Some influencing factors on ISSR-PCR analysis, such as Mg^2＋, dNTPs, Taq polymerase, template DNA and primer, were selected and optimized, using Amygdalus Ledebouriana Schleche as materials, The optimal ISSR-PCR reaction system for A, Ledebouriana Schleche was determined as follows： 1 × buffer, 2. 0 mmol · L^1 Mg^2＋ and 160 ·mol · L^-1 dNTPs, 30 ng template DNA, 1U Taq polyme, ase. 15 pmol primer in 25μL PCR reaction system.