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PCR-DGGE技术解析针叶和阔叶凋落物混合分解对土壤微生物群落结构的影响
  • ISSN号:1006-687X
  • 期刊名称:《应用与环境生物学报》
  • 时间:0
  • 分类:S154.3[农业科学—土壤学;农业科学—农业基础科学]
  • 作者机构:[1]中国科学院生态环境研究中心 城市与区域生态国家重点实验室,北京100085, [2]海南省环境科学研究院,海口570206, [3]湖南农业大学农学院,长沙410128
  • 相关基金:国家自然科学基金项目(Nos.40871130 30600474)资助
中文摘要:

为深入理解凋落物类型和微生物群落之间的相互关系,通过小盆+凋落袋控制实验,运用PCR-DGGE技术解析了南方红壤丘陵区典型针叶树种马尾松和湿地松的凋落物分别与白栎、青冈两个阔叶树种凋落物混合分解对土壤微生物基因型多样性的影响.结果表明:1)针叶凋落物中加入阔叶后细菌群落结构未发生显著变化,将所有处理归为一类的相似度达72%,其差异仅表现在马尾松+白栎和湿地松+青冈处理土壤微生物群落16S rDNA的丰富度和多样性分别显著高于单一马尾松和湿地松;2)针叶凋落物中加入阔叶后真菌群落结构发生了显著变化,单一针叶处理与针阔混合处理间18S rDNA基因泳道带型的相似度只有28%,并且单一马尾松处理土壤18S rDNA的丰富度和多样性显著高于马尾松+白栎和马尾松+青冈,单一湿地松处理土壤18S rDNA的丰富度也显著高于湿地松+白栎和湿地松+青冈,多样性显著高于湿地松+白栎处理;3)土壤真菌18S rDNA的丰富度与凋落物初始C含量呈显著正相关,与凋落物初始N含量呈显著负相关.针叶凋落物中引入阔叶凋落物后,增加了凋落物中N的比重,C的比重则下降,显著影响了土壤真菌群落的结构.

英文摘要:

Clarifying the microbiological mechanism of litter decomposition is of great significance,especially in understanding how the soil microbial communities affect the decomposition of mixed litters with conifer and broadleaf trees.In this study,a simulation experiment was designed with flowerpots,and the litters of the typical species in the southern hilly red soil region,including Pinus massoniana(PM),Pinus elliottii(PE),Quercus fabri(QF) and Cyclobalanopsis glauca(CG),were investigated as study materials.The effects of the decomposition of the mixed litters on the genotypic diversity of soil microbial communities were studied by using PCR-DGGE method.The results indicated: 1) No significant change occurred in bacterial community structure and the lowest similarity was 72% if all treatments were graded as one class after mixing broadleaf litter with conifer litter.The differences were that the richness and diversity of 16S rDNA in PM+QF and PE+CG were significantly higher than that of single-conifer litter of PM and PE,respectively;2) Fungal community structure significantly changed and the similarity between the single-conifer litter treatment and the mixed litter treatment was only 28%.The richness and diversity of 18S rDNA in PM were significantly higher than those of PM+CG.The richness of 18S rDNA in PE was also significantly higher than that of PE+QF and PE+CG,and the diversity was significantly higher than that of PE+QF;3) The richness of 18S rDNA was positively related with initial carbon content of litter,but significantly negative with initial nitrogen content of litter.After mixing broadleaf litter with conifer litter,the proportion of nitrogen in litters increased,but the proportion of carbon declined significantly,which significantly affected the soil fungal community structure.

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期刊信息
  • 《应用与环境生物学报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学院
  • 主办单位:中国科学院成都生物研究所
  • 主编:吴宁
  • 地址:成都市人民南路4段9号
  • 邮编:610041
  • 邮箱:biojaeb@cib.ac.cn
  • 电话:028-82890917
  • 国际标准刊号:ISSN:1006-687X
  • 国内统一刊号:ISSN:51-1482/Q
  • 邮发代号:62-15
  • 获奖情况:
  • 中国农业期刊金犁奖学术类一等奖(2006),* 中国期刊方阵双效期刊(2002年),* 四川省第二届优秀期刊(2000年),* 四川省一级学术期刊(1998年)
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),荷兰文摘与引文数据库,美国生物科学数据库,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版)
  • 被引量:23016