应用Biolog方法研究了不同森林恢复类型(人工恢复的湿地松林、杉木林、油茶林和自然恢复的天然次生林)土壤微生物群落对Biolog—GN板不同类型碳源的利用情况,结果表明,自然恢复的天然次生林土壤微生物群落的碳源代谢能力比3种人工林强,其次是油茶林.4种森林类型土壤微生物群落比较偏好、利用率较高的3类碳源是糖类、羧酸类和氨基酸类.对4类森林恢复类型土壤微生物群落具有分异作用的主要碳源类型亦为糖类、羧酸类和氨基酸类,这3类碳源是研究森林恢复后导致土壤微生物群落变化的敏感碳源.
Utilization of different carbon source type in Biolog-GN microplates by soil microbial communities under different forest restoration types was studied. The results shows that metabolic capacity of soil microbial commuinty under natural secondary forest are higher than those of three plantations. Carbohydrates, carboxylic acids and amino acids are the main carbon sources possessing higher utilization efficiency or utilization intensity. At the same time, the three carbon source types contributed to the differentiation of soil microbial communities from four forest restoration types. And the three types of carbon sources were sensitive to change of soil microbial communities. These results possessed important referenced significance for substrate selection during the study on soil microbial communities and their functional diversity with incubating methods.