中文摘要：

目的 探讨甘草酸二铵（DG）对胰腺癌Bx PC-3细胞株的影响。方法 胰腺癌Bx PC-3细胞株随机分成对照组,5-氟尿嘧啶（5-FU）组（50μg/ml）,DG高、低剂量组（48,24μg/ml）。采用四甲基偶氮唑蓝比色法（MTT）检测胰腺癌Bx PC-3细胞株的增殖抑制率;采用细胞划痕法检测胰腺癌Bx PC-3细胞株的运动能力;电子显微镜观察胰腺癌Bx PC-3细胞株镜下形态结构;采用免疫组化和RT-PCR检测胰腺癌Bx PC-3细胞株中Raf-1水平。结果 与对照组比较,5-FU组及DG高、低剂量组细胞OD值明显降低（P〈0.05）,运动能力明显降低（P〈0.05）,胰腺癌Bx PC-3细胞株形态结构明显损伤,Raf-1水平明显降低（P〈0.05）。结论 DG通过抑制Raf-1在胰腺癌治疗中发挥重要作用。

英文摘要：

Objective To study the effects of diammonium glycyrrhizinate（DG） on BxPC-3 cell line of pancreatic cancer. Methods BxPC-3 cell lines of pancreatic cancer were randomly divided into control ,5-Fluorouracil（ 5＇-FU, 50 μg/ml）, DG high-dose （48 μg/ml ）, DG low-dose （24 μg/ml） groups. The MTT was used to determine OD values of BxPC-3 cell line of pancreatic cancer. The cell scratch was used to determine the exercise capacity of BxPC-3 cell line of pancreatic cancer. Morphology of BxPC-3 cell line of pancreatic cancer was ob- served by optical electron microscopy. The immunohistochemistry and real-time-PCR were applied for detecting rapidly accelerated fibrosareo- ma（Raf） -1 levels in BxPC-3 cell line of pancreatic cancer. Results Compared with that of control group, OD values of BxPC-3 cell line of pancreatic cancer in 5-FU, DG high-dose and DG low-dose groups were significantly decreased（ P〈0.05 ）, exercise capacity were decreased （ P〈0.05 ）. Compared with those of control group, BxPC-3 cell line of DG high-close and medial-dose groups were damaged normal morpho- logical structure, Raf-1 level was significantly decreased（ P〈0.05 ）. Conclusions DG plays a certain role in the treatment of BxPC-3 cell line of pancreatic cancer through inhibiting Raf-1.