中文摘要：

目的：探讨短期和长期运动预适应对大鼠血液和心脏降钙素基因相关肽（calcitonin gene-relatedpeptide,CGRP）的影响及其对心肌保护的作用机制;方法：健康雄性SD大鼠75只,随机分为对照组（C组）、3天运动预适应组（3dEP组）和3周运动预适应组（3周EP组）。3dEP组和3周EP组分别进行3天和3周的间歇跑台训练建立短期和长期运动预适应动物模型。用酶联免疫法检测血清CGRP含量的变化,用western blot方法和免疫组织化学方法检测心脏CGRP定量和定位表达的变化;结果：与C组比较,3dEP组血清CGRP含量变化不明显,3周EP组血清CGRP含量明显升高（P〈0.05）;与C组比较,3dEP组心脏CGRP表达明显减少,CGRP免疫反应阳性表达面积和平均光密度明显降低（P〈0.05）,3周EP组心脏CGRP表达变化不明显,CGRP免疫反应阳性表达面积和平均光密度无明显变化;结论：短期运动预适应使心脏CGRP表达下降,提示短期运动预适应可促进心脏CGRP的释放,对心肌产生保护作用;长期运动预适应使血清CGRP水平升高,提示长期运动预适应可提高机体CGRP的释放水平,对心肌产生保护作用。

英文摘要：

Objective：The effects of short-term and long-term exercise preconditioning on calcitonin gene-related peptide （CGRP） in the blood and heart of rats and the mechanism of cardioprotection were studied.Method：Seventy five healthy male SD rats were randomly divided into control group （C）,3 days （3 d EP） and 3 weeks （3 w EP） exercise preconditioning groups.3 d EP and 3 w EP groups performed 3 days and 3 weeks interval treadmill exercise respectively in order to establish short-term and long-term exercise preconditioning animal models.The content of CGRP in serum was detected with enzyme immunoassay method.The expression of CGRP in heart was showed by western blot and immunohistochemistry.Result：As compared with the group C：the content of serum CGRP changed insignificantly in group 3 d EP,the content of serum CGRP increased significantly in group 3 w EP （P0.05）; the expression of cardiac CGRP decreased significantly in group 3 d EP,the positive area and optical density of CGRP immunoreaction were significant lower in group 3 d EP （P0.05）,the expression of cardiac CGRP changed insignificantly in group 3 w EP,the positive area and optical density of CGRP immunoreaction changed insignificantly in group 3 w EP.Conclusion：Short-term exercise preconditioning can decrease the expression of CGRP in heart,which demonstrated myocardial protection is mainly associated with the increase of CGRP released in heart.Long-term exercise preconditioning can improve the level of CGRP in serum,which demonstrated myocardial protection is mainly associated with the increase of CGRP released in body.