中文摘要：

目的：探讨运动预适应对大鼠背根神经节降钙素基因相关肽（calcitonin gene-related peptide,CGRP）表达的影响。方法：健康雄性Sprague-Dawley大鼠50只,随机分为对照组和运动预适应组。运动预适应组进行连续3周的间歇跑台训练建立运动预适应动物模型。用实时荧光定量PCR技术检测背根神经节CGRP mRNA的表达,用免疫组织化学SABC法观察背根神经节CGRP的表达。结果：运动预适应组背根神经节CGRP mRNA表达与对照组相比变化不明显（P〉0.05）;运动预适应组背根神经节CGRP免疫反应与对照组相比明显增强,CGRP免疫反应阳性表达面积和平均光密度均显著高于对照组（P〈0.05）。结论：运动预适应未引起背根神经节CGRP mRNA的表达发生明显变化;运动预适应使背根神经节CGRP的表达增强,以促进外周神经末梢CGRP的储备和释放,介导运动预适应的内源性保护作用。

英文摘要：

Objective The goal of this study was to explore the effect of exercise preconditioning on the expression of calcitonin gene-related peptide（CGRP）in the dorsal root ganglion of rats.Methods Fifty healthy male SD rats were randomly divided into control group（C）and exercise preconditioning group（EP.） Group EP performed interval treadmill exercise for 3 weeks for establishing exercise preconditioning animal model.The expression of CGRP mRNA in dorsal root ganglion was investigated by real-time fluorescence quantitative PCR.The immunoreaction of CGRP in dorsal root ganglion was shown by immunohistochemistry.Results There was no significant difference in the expression of CGRP mRNA in two groups（P 0.05）.As compared with the group C,the immunoreaction of CGRP was increased in group EP,and the positive area and mean optical density of CGRP immunoreaction in group PE were significant higher than that in group C（P0.05）.Conclusion Exercise preconditioning does not change the expression of CGRP mRNA in dorsal root ganglion,but enhances the expression of CGRP in dorsal root ganglion to promote the reserve and release of CGRP in peripheral nerve endings and has the same endogenous protection as ischemic preconditioning.