中文摘要：

目的：研究巴氯芬及β-环糊精的包结作用和巴氯芬及β-环糊精包结物与牛血清白蛋白的相互作用。方法：用紫外可见分光光度法与核磁共振法研究了β-环糊精对巴氯芬的包结行为;用摩尔比法确定了包结物的化学计量比;用公式计算了不同温度下巴氯芬与β-环糊精的包结常数;用荧光光谱法研究了巴氯芬与包结物对牛血清白蛋白牛血清白蛋白的相互作用。结果：巴氯芬与β-环糊精的包结过程是自发的,主要驱动力为疏水作用力;巴氯芬及包结物都会对牛血清白蛋白产生静态猝灭。结论：巴氯芬与β-环糊精的包结比为1∶1;巴氯芬与牛血清白蛋白的主要作用为疏水作用,而包结物与牛血清白蛋白主要为静电作用,这是由于β-环糊精将巴氯芬分子中疏水部分包结的结果。

英文摘要：

Objective：The inclusion interaction of β-cyclodextrin and baclofen,and the interactions of bovine serum albumin and baclofen or complex were investigated.Methods：The inclusion interaction of β-cyclodextrin and baclofen was studied by UV spectra and H1NMR.The stoichiometry ratio for the formation of the inclusion complexes was determined by molar ratio method.The constants of baclofen and β-cyclodextrin at different temperatures were estimated according to the formula.The interactions of bovine serum albumin and baclofen or complex have been studied by fluorescence spectroscopy.Results：The result showed that the inclusion process was spontaneous,the hydrophobic force was main binding force of β-cyclodextrin inclusion complex;Both baclofen and complex could quench the fluorescence of bovine serum albumin.Conclusion：The stoichiometry ratio for the formation of the inclusion complexes is 1∶1.The hydrophobic force of baclofen alone and bovine serum albumin is mainly interaction force,and the electrostatic force of inclusion compond and bovine serum albumin is mainly interaction force,for the reason that baclofen is included by the β-cyclodextrin.