中文摘要：

目的利用4种不同支架材料构建复合式口腔黏膜,并比较其组织结构特点。方法体外培养人口腔黏膜的成纤维细胞和角质形成细胞,在4种支架材料中加入成纤维细胞,培养7d后,在支架表面加入角质形成细胞,培养4d后,移至气-液界面继续培养7d。苏木精-伊红染色镜下观察构建的复合式口腔黏膜的组织形态学特点。结果 4种支架均可构建形成复层上皮。其中,上皮层与脱细胞真皮基质材料（de-epidermised dermis,DED）结合紧密,形成的人工黏膜有明显的上皮钉突。不同于以往报道,上皮层与Alloderm结合并不十分紧密。以胶原凝胶为基质形成的人工口腔黏膜最厚,有明显分层。以胶原海绵-胶原凝胶为基质形成的复层上皮在部分区域长入至胶原海绵的空隙中。结论以DED和胶原凝胶为支架构建的口腔黏膜更接近于天然结构,而后者脆性较大,限制了其临床应用的可能。

英文摘要：

Objective To develop,optimize and characterize tissue engineered oral mucosa equivalents made of an epithelium layer on 4 different scaffolds. Methods Fibroblasts and keratinocytes were isolated and cultured from mucosa biopsies obtained from healthy volunteers.After amplification,fibroblasts were seeded into de-epidermized human dermis（DED）and Alloderm by centrifugation.The collagen based scaffolds were prepared by seeding fibroblasts directly.After one week culture,the keratinocytes were seeded on top of the dermis and cultured for another 11 days.The equivalents were all prepared for histology. Results General histology showed that multilayered,stratified and differentiated epithelia were formed on all 4 different scaffolds.Oral epithelia cultured on DED closely attached to the dermis,and formed obvious rete pegs resembling normal oral epithelium.Different from previous reports,the epithelia cultured on the Alloderm did not attach tightly to the substrate.The epithelia generated on the fibroblast-populated collagen scaffold showed a fully stratified structure with apparent cornification.The epithelia formed on the collagen sponge-collagen gel complex invaded into the small gaps that were not filled with collagen gel. Conclusion Oral keratinocytes cultured on DED and collagen gel exhibited tissue organization that resembled normal oral mucosa.For the latter one limited clinical use is possible due to dramatic shrinkage and reduced stability.