中文摘要：

目的研究肝细胞癌患者免疫抑制性Treg细胞对CD8＋T淋巴细胞穿孔素表达的影响。方法采集20例肝细胞癌患者和20名健康人的外周血,用流式分析法检测抗-CD3/CD28刺激48 h后CD8＋T淋巴细胞的穿孔素表达情况。免疫磁珠分离法分离健康人CD8＋T淋巴细胞和肝细胞癌患者Treg细胞,一组CD8＋T淋巴细胞单独培养,另一组CD8＋T淋巴细胞与Treg细胞共同培养,用流式细胞法检测抗-CD3/CD28刺激48 h后两组CD8＋T淋巴细胞穿孔素表达情况。结果肝细胞癌患者外周血中CD8＋T淋巴细胞穿孔素表达量与健康人相近,分别为（10.74±3.96）%和（12.6±2.48）%,差异无统计学意义（P〉0.05）。CD8＋T淋巴细胞单独培养和与肝癌患者Treg细胞共培养后,健康人CD8＋T淋巴细胞穿孔素表达量分别为（34.2±3.65）%和（20.43±4.52）%,差异有统计学意义（t=11.42,P〈0.01）。结论肝癌患者免疫抑制性Treg细胞可使CD8＋T淋巴细胞穿孔素表达量降低。

英文摘要：

Objective To investigate the influence of immunosuppressive regulatory T cells （Treg）in patients with hepatocellular carcinoma （HCC）on perforin expression of CD8 ＋ T lymphocytes.Methods Perforin expression of CD8 ＋ T lymphocytes in 20 healthy subjects and 20 HCC patients was detected by flow cytometry.CD8 ＋ T lymphocytes from 20 healthy subjects and Treg from 20 HCC patients were collected by magnetic activated cell sorting （MACS）,then were divided into two groups：single culture of CD8 ＋ T lymphocytes and co-culture of CD8 ＋ T lymphocytes and Treg.Both groups were stimulated by anti-CD3/CD28 for 48 hours.Perforin expressions of CD8 ＋ T lymphocytes in both groups were quantitatively detected by flow cytometry.Results Perforin expression of CD8 ＋ T lymphocytes in HCC patients （10.74%＆±3.96%）was similar with that in healthy subjects （12.6% ＆±2.48%）（P 〉0.05 ）.However,perforin expression was significantly decreased after CD8 ＋ T lymphocytes were co-cultured with Treg [（34.2%＆±3.65%）and （20.43%＆±4.52%）, respectively （P 〈0.01）].Conclusion Treg could significantly reduce perforin expression of CD8 ＋ T lymphocytes.