中文摘要：

目的观察穿心莲内酯对热休克蛋白70（HSP70）基因表达的影响，探讨其抗应激作用机制。方法利用RT—PCR和Western blot方法分别检测穿心莲内酯对HeLa细胞中HSP70mRNA和蛋白表达的影响；采用双荧光素酶报告基因技术，检测穿心莲内酯对HSPT0启动子活性的影响。结果RT—PCR和Westernblot分析显示穿心莲内酯可诱导HSP70mRNA和蛋白表达增多，同时引起热休克因子1的磷酸化修饰；报告基因技术显示穿心莲内酯可增强HSP70转录活性。结论穿心莲内酯具有抗应激作用，其作用机制可能与增加HSP70表达有关。

英文摘要：

Objective To evaluate effects of andrographolide on the expression of heat shock protein 70 （HSP70） in HeLa cell line. Methods Effects of andrographolide on expression and mRNA of HSP70 in HeLa cells were assessed by Western blot and RT - PCR. Dual luciferase reporter assay was used to study effect of andrographolide on the transcriptional activity of HSP70. Results Andrographolide induced up - regulation of the mRNA and protein of HSP70 ; also enhanced the phosphorylation of HSP1, which enhanced the transcription of HSP70. Conclusion Andrographolide has the anti - stress effect, which is probably mediated with up - regulation of HSP70. words]