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杂交稻幼苗期对低温胁迫的生理反应
  • ISSN号:0496-3490
  • 期刊名称:《作物学报》
  • 时间:0
  • 分类:S511[农业科学—作物学]
  • 作者机构:[1]南京师范大学生命科学学院,江苏南京210097, [2]皖南医学院,安徽芜湖241000, [3]江苏省农业科学院粮食作物研究所,江苏南京210014
  • 相关基金:国家自然科学基金(30270792)、江苏省自然科学基金(BK2004143)和江苏省博士后科研资助计划.
中文摘要:

研究了常规低温敏感型杂交稻汕优63和超高产杂交稻两优培九幼苗期对低温胁迫的抗性,处理低温为10℃,以常温25℃处理为对照。结果表明,随低温处理时间的延长,水稻幼苗叶片叶绿索含量逐渐下降、类囊体膜PSⅠ、PSⅡ活性明显下降,类囊体膜的室温吸收光谱、发射光谱也明显下降。低温使两优培九的O2^-产生速率和MDA含量下降,而汕优63却出现相反的结果;同时,SOD的活性与对照相比两品种都有所提高,而CAT活性则是两优培九高于对照,汕优63低于对照。这些指标的变化幅度都是汕优63较明显。低温处理后,两品种的可溶性蛋白含量都上升,但两优培九增幅较汕优63高。SDS-PAGE表明,低温处理后可溶性蛋白条带增多,但类囊体膜蛋白条带没有明显的增加。对照组中,除了PSⅠ和PSⅡ活性外,其余各项指标都是汕优63较两优培九高,意味着幼苗期的两优培九光合功能虽不如汕优63高,但其对低温的抗性较汕优63强。

英文摘要:

To clarify the resistance of high-yield hybrid rice ( Oryza sativa L. ) at seedling stage to chilly temperature, the photosynthetic characters and protein component of two cultivars, a newly-developed cultivars super-hybrid rice Liangyoupeijiu and a traditional chill-sensitive hybrid rice Shanyou 63, were investigated by setting 25℃ as control and 10℃ as low temperature treatment. The results showed that the chlorophyll contents, the consuming oxygen activity of photosystem Ⅰ (PS Ⅰ ) and oxygen evolution activity of photosystem Ⅱ (PS Ⅱ ) of thylakoid membrane were decreased obviously in low temperature treatments, so were the absorption spectrum and the emission spectrum of thylakoid membrane at room temperature. The contents of O2^- and MDA of Liangyoupeijiu were decreased slightly, but these of Shanyou 63 were increased. Chilly temperature caused an increase of the activity of SOD compared with the control in two cultivars. As compared with the control, CAT activity was higher in Liangyoupeijiu and lower in Shanyou 63. All the indexes for Shanyou 63 showed a more distinctive dropping trend. After chilly treatment, the soluble protein content in two cultivars especially in Liangyoupeijiu was significantly higher than those of control. The SDS-PAGE of soluble protein revealed that the protein bands were increased during chilly treatment, but this change did not occur in thylakoid membrane proteins. In the control, almost all parameters investigated except the activities of PS Ⅰ and PS Ⅱ were higher in Shanyou 63 than in Liangyoupeijiu, which means that at the seedling stage, Liangyoupeijiu may have stronger resistance to chill-stress than Shanyou 63 although the latter has higher photosynthetic function.

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期刊信息
  • 《作物学报》
  • 北大核心期刊(2011版)
  • 主管单位:中国科学技术协会
  • 主办单位:中国作物学会 中国农业科学院作物科学研究所
  • 主编:万建民
  • 地址:北京海淀区中关村南大街12号中国农业科学院
  • 邮编:100081
  • 邮箱:zwxb301@caas.cn
  • 电话:010-82108548
  • 国际标准刊号:ISSN:0496-3490
  • 国内统一刊号:ISSN:11-1809/S
  • 邮发代号:82-336
  • 获奖情况:
  • 2002年-第三届中国科协优秀科技期刊二等奖,2002-2009年-百种中国杰出学术期刊,2004年获“全国优秀期刊一等奖”,2005年获第三届国家期刊奖提名奖,2009年评为“2008年度中国精品科技期刊”,2009年被评为“新中国60年有影响力的期刊”,2011年获"第二届中国出版政府奖期刊奖提名奖"
  • 国内外数据库收录:
  • 俄罗斯文摘杂志,美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,美国剑桥科学文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:49369