中文摘要：

目的采用大鼠去卵巢合并D-半乳糖诱导老年性骨质疏松的动物模型来评价从红曲提取含辅酶Q10和洛伐他汀的提取物对胫骨形态的骨计量学作用,并与雌激素己烯雌酚比较。方法 3月龄SPF级雌性SD大鼠,随机分为假手术对照组（CON）,去卵巢组（OVX）,模型组（MOD）,己烯雌酚组（DES）和红曲胶囊组（RYR）,连续给药60天。实验结束前第13、14天和第3、4天皮下注射钙黄绿素7 mg·kg^-1进行骨荧光标记。实验结束时,取右侧胫骨上段制成不脱钙骨切片,胫骨中段制成不脱钙骨磨片用于骨组织形态计量学研究。结果去卵巢合并皮下注射D-半乳糖可导致大鼠体重增加,胫骨松质骨骨量丢失严重,骨显微结构严重退化,胫骨皮质骨的面积减少;含辅酶Q10和洛伐他汀的红曲提取物可使去卵巢合并D-半乳糖大鼠的体重明显减轻,胫骨松质骨以及胫骨皮质骨的骨丢失明显减轻,骨量、骨微观结构得到明显改善。结论去卵巢合并D-半乳糖可导致大鼠出现老年性骨质疏松,补充含辅酶Q10和洛伐他汀的红曲提取物可改善去卵巢合并D-半乳糖大鼠的胫骨骨丢失,提示该提取物具有减肥及防治骨质疏松的良好的应用前景。

英文摘要：

Objective To establish the model of osteoporosis induced by OVX and D-galactose in rats,to investigate the effect of red yeast rice extract components containing coenzyme Q10 and lovastatin on the tibia,and to compare the effect with that of diethylstilbestrol. Me thods Three-month-old female Sprague-Dawley rats were randomly into sham group（ CON）,ovariectomized group（ OVX）,model group（ MOD）,diethylstilbestrol group（ DES）,and red yeast rice capsule group（ RYR）.All rats were weighed weekly and received subcutaneous injection of calcein（ 7 mg·kg^- 1） on day 14,day 13,day 4,and day 3before sacrificed. After 60 days,static and dynamic histomorphometric analysis on the cancellous bone of the proximal tibia metaphysis（ PTM） and tibia shaft（ Tx） was measured in undecalcified sections. Re sults After OVX and D-galactose injection,the body weight and the bone loss of cancellous bone in the tibia increased in rats,and the cortical bone in tibia reduced significantly. In addition,serious degradation of the bone microstructure was observed in the rats. Red yeast rice extract components containing coenzyme Q10 and lovastatin effectively prevented body weight gain and the bone loss of cancellous bone and the cortical bone in the tibia in rats. The bone mass and bone microstructure also improved. Conclusion OVX and D-galactose injection lead to osteoporosis in rats. Red yeast rice extract components containing coenzyme Q10 and lovastatin prevent the tibia bone loss in rats treated with OVX and D-galactose,suggesting a good prospect of the components in weight loss and anti-osteoporosis application.