中文摘要：

目的：肿瘤坏死因子-α（tumor necrosis factor-α，TNF-α）基因启动子单核苷酸多态性与某些炎性疾病、肿瘤的发生有关。本研究就TNF-α-308G/A位点基因多态性与中国人群原发性肺癌易感性进行探讨。方法：应用高通量TaqMan-MGB探针技术对TNF-α-308G/A位点，即rs1800629位点进行基因分型，分析比较447例健康对照者和250例原发性肺癌患者的基因类型。采用SPSS 18.0软件对数据资料进行统计分析。结果：TNF-α-308G/A位点GG基因型在病例组和对照组中的频率分别为73.2%和90.8%。A/G基因型在病例组和对照组中频率分别为26.8%和8.7%。基因型A/G＋AA频率分别为26.8%和9.2%，该位点多态性在病例组和对照组中的分布频率差异具有统计学意义（P〈0.05）。此外，A/G基因型在男性、吸烟、小细胞肺癌或非小细胞肺癌患者相对于GG型均为危险因素，但在女性中无统计学意义（P〉0.05）。结论：TNF-α-308G/A位点基因多态性与中国原发性肺癌发病易感性显著相关。

英文摘要：

Objective：Previous studies suggested that the-308G/A allele in the tumor necrosis factor-α（TNF-α） gene promoter （-308G/A） may be a potential risk factor for inflammatory diseases and tumor progression. However, only a few studies have focused on the-308 polymorphism of TNF-αgene with primary lung cancer in Chinese population. This study aims to evaluate the role of TNF-α-308G/A single nucleotide polymorphism （SNP） and the risk of primary lung cancer in Chinese population. Methods：A total of 250 patients and 447 healthy individuals （control group） were involved in this study. Genotyping was performed using TaqMan technology. Results：The frequencies of （GG）, （A/G）, and （A/G＋AA） genotypes of-308G/A SNP in TNF-αgene were 183 （73.2%）, 67 （26.8%）, and 67 （26.8%） in the patients, and 406 （90.8%）, 39 （8.7%）, and 41 （9.2%） in the control group, respectively. The distribution of poly-morphism frequencies in the case group and the control group showed a statistically significant difference for the Chinese population （P〈0.05）. Conclusion：Results indicated that TNF-αgene polymorphism at position-308G/A is associated with susceptibility to lung cancer in Chinese Han population.