中文摘要：

目的探讨PTD-HBcAg融合蛋白诱导小鼠体内特异性CTL并抑制HepG2.2.15细胞HBV复制的作用。方法 PTD-HBcAg、HBcAg和阴性对照分别与等体积的弗氏佐剂乳化后皮下免疫小鼠;第14d,分离脾淋巴细胞并分别用PTD-HBcAg、HBcAg、PTD和PBS加强刺激后收集上清,检测细胞因子IFN-γ、IL-12、IL-4和IL-10;刺激后的淋巴细胞作为效应细胞与HepG2.2.15细胞共培养,检测,效应细胞对HBsAg、HBVDNA的抑制作用及对HepG2.2.15细胞、HepG2细胞的杀伤效果。结果 PTD-HBcAg组分泌的IFN-γ、IL-12、IL-4和IL-10与HBcAg组和阴性对照组比较差异有统计学意义（P〈0.05）;PTD-HBcAg融合蛋白组较HBcAg组和阴性对照组有更明显的病毒抑制作用（P〈0.05）;PTD-HBcAg组对HepG2.2.15细胞的杀伤率明显高于HBcAg组和阴性对照组（P〈0.05）。结论 PTD-HBcAg可诱导HBV特异性CTL,能有效抑制HepG2.2.15细胞HBV的复制。

英文摘要：

Objective To study the effects of PTD-HBcAg inducing HBV specific cytotoxic T lymphocytes in mice on inhibiting HBV replication in HepG2.2.15 cells. Methods BALB/c mice were subcutaneously immunized at the tail base with 100μl of freshly prepared emulsion containing with PTD-HBcAg,HBcAg,PTD,PBS and an equal volume of CFA. 14days later,T lymphocytes in spleen were isolated and stimulated with corresponding proteins to detect the level of IFN-Υ,IL-12,IL-4 and IL-10 in the supernatants. The stimulated T lymphocytes as effective cells were cocultured with HepG2.2.15 cells to determine the levels of HBsAg and HBV DNA and to observe the cytotoxicity of effective cells to HepG2.2.15 cells. Results The cytokines in PTD-HBcAg groups were markedly higher than HBcAg groups and normal controlled groups;In comparison with normal controlled groupsand HBcAg groups,HBsAg and HBV DNA decreased more obviously in PTD-HBcAg groups;The T lymphocytes stimulated by PTD-HBcAg could kill HepG2.2.15 cells more effectively than others. Conclusions PTD-HBcAg can highly promote HBV specific CTL to inhibit HBV replication in HepG2.2.15 cells.