中文摘要：

目的：检测缺氧诱导因子-1α（HIF—1α）、P-糖蛋白在腋淋巴结阴性乳腺癌组织中的表达情况及其与乳腺癌分型、分级及预后的关系。方法：采用免疫组织化学方法检测81例乳腺癌和20例癌旁组织中HIF-1α、P—gp的表达状况，分析与各临床病理因素和预后的关系。结果：HIF—1α在乳腺癌中总阳性表达率为69．1％．其中强表达10例（123％），中度表达12例（14．8％），弱表达34例（42．0％），隐性25例（30．9％）；81例乳腺癌组织中34例P—gp表达阳性，占42．0％，其中强表达7例（8．6％），中度表达9例（11．1％），弱表达18例（22.2％），阴性47例（58．0％）HIF—1α和P—gp在乳腺癌中两者阳性指数呈正相关（r=-0．62，P〈0．01）HIF—1α为单独影响乳腺癌预后的危险因素结论：HIF—1α和P—gp两者阳性指数在乳腺癌中呈正相关；HIF—1α可作为判断乳腺癌恶性程度、预后的单独指标，高表达者5年生存率低，检测HIF—1α有助于提高评估腋淋巴结阴性乳腺癌术后生存的准确性。

英文摘要：

Objective： To study the expression of HIF-1α and P-gp in breast cancer and to determine the correlation with prognosis. Methods： Formalin-fixed, paraffin-embedded samples from 81 breast cancer patients were tested for HIF-1α and P-gp expression by immunocytochemical staining. Then the influence of these expression levels on the clinical and pathological parameters and on survival was investigated. Results： Strong expression of HIF-1α was observed in 10 cases （12.3%）, while expression was moderate in 12 cases （14.8%） and weak in 34 cases （42.0%）. No expression was found in 25 cases （30.9%）. Strong expression of P-gp was observed in 7 cases （8.64%）, moderate expression was observed in 9 cases （11.1%） and weak expression was observed in 18 cases （22.2%）. No expression was found in 47 cases （58.0%）. HIF-1α expression had a significant positive correlation with the expression of P-gp （r=0.62, P〈0.01）. In the 64 cases with follow-up data, univariate analysis revealed that the survival rate was correlated with histological grading, staging, tumor size, and expression of HIF-1α and P-gp. However, Cox stepwise proportional hazards analysis showed that only HIF-1α was considered independently significant for predicting patient outcome. Conclusions： The expression of P- gp may be correlated with HIF-1α expression. HIF-1α expression is correlated with the prognosis of breast carcinoma. The expression of HIF-1α can be used to accurately assess the prognosis in breast carcinoma and may be helpful for evaluating the prognosis of ANNBC.