中文摘要：

目的检测βcatenin在大鼠髋脱位股骨头软骨浅层中的动态表达，探讨βcatenin与髋脱位关节软骨早期退变以及与在不同应力区域表达的关系。方法选取新生Wistar大鼠80只，随机分成髋脱位组（n=40）和对照组（n=40），持续固定10d建立新生大鼠髋脱位模型后去除外固定，分别于鼠龄第2、4、6、8周处死、离断髋关节，用于测量组织形态和βcatenin免疫组化，应用qRTPCR检测股骨头软骨中βcatenin的mRNA表达。结果①成功制作髋脱位动物模型，髋臼指数及股骨头指数在不同时段表现出显著差异（P＜0001）。②髋脱位组镜下可见软骨排列紊乱，后期出现软骨表面裂隙以及溃疡；Mankin评分早期无显著性差异；于第4、6、8周表现出显著差异。股骨头浅层软骨βcatenin的表达于第2周时实验组明显高于对照组；第4周时实验组明显低于对照组；第6、8周实验组表达显著增多。髋脱位组中Mankin评分与βcatenin之间有相关关系。βcatenin的mRNA表达在不同时段均有显著差异，在对照组中呈现逐渐下降趋势，而在实验组中却逐渐上调。结论βcatenin在髋脱位股骨头浅层软骨的发育和退变中发挥着双向调控作用，可能与异常应力的作用有着密切关系。

英文摘要：

Objective To explore the roles of β-eatenin in the development and early degeneration of the dysplastic hip by examining its expression in superficial zones of femoral head. Methods 80 neonatal Wistar rats were randomly divided into developmental dislocation of the hip（DDH） group and control group. The DDH model was induced by fixation of both hips and knees at extension for ten days. The hips were isolated at 2 - , 4- ,6- ,8 -, week- old for gross morphometry and immunoehemistry of β-catenin. Meanwhile, mRNA of β- catenin in cartilage of femoral head was extracted and assessed by qRT-PCR, Student＇s t -tests were used for comparison between two groups, with anova for the relationship between Mankin score and β-catenin expression. Results first ： the model was successfully identified by presentation of coronal anatomical relationship. The acetabular index and femoral head index at DDH group showed much smaller than normal hip joint at each period. Meanwhile, the dis - organized chondrocytes and clefts in the superficial zones were observed with high Mankin score at later stages,while the expression of β-catenin Was strongly relative to the degrees of degeneration with articular cartilage got close to maturity. Accordingly, the mRNA expression of β-catenin in superficial zone at 2 weeks old was obviously up regulated in DDH group compared to normal cartilage, furthermore, it showed up-regulation again adjacent to maturity. Conclusion β-catenin in the superficial zones of cartilage may play dural roles not only in the development but in degenenration of dysplastic hip, and it may be attributed to the influence of the abnormal loading on femoral head.