通过室内控制实验,研究了不同曝气比率对蛋白核小球藻(Chlorella vulgaris)生长过程影响,构建了曝气比率与ODmax、μmax、Cmax的适配曲线。实验设置0%、2%、10%、20%、30%、50%、70%共计7组曝气比率,在1000 lx光强和20℃条件下,采用BG-11培养基培养小球藻至稳定生长。结果显示,适宜的曝气能促进小球藻生长,其最适曝气比率为20%,过量曝气会抑制小球藻生长;曝气比率(x)与 ODmax、Cmax、μmax拟合方程分别为:Omax =170.63x3-231.83x2+84.341x +1.8439(0<x<50%;R2=0.9850)、Cmax =15.844x3-19.803x2+6.8594x+0.0521(0<x <50%;R2=0.9285)、μmax =8.1202x3-11.428x2+4.4963x +0.1173(20%<x <30%;R2=0.8581);50%<x<70%的关系式有待进一步验证。探究不同曝气比率对小球藻生长的影响,可为其优化培养与资源化利用提供理论依据。
The study aimed to investigate the effects of varying aeration rates (0%,2%,10%,20%,30%, 50%,and 70%)on the growth of Chlorella vulgaris.The experiment was conducted under 1000 lx light intensity at 20℃,and the Chlorella vulgaris was cultivated on the BG-1 1 culture medium to stable condition.Fitted curves between aeration ratio and the maximum optical density (ODmax),the maximum growth rate (μmax)and the maxi-mum concentration of daily biomass (Cmax)were created.The results showed that appropriate aeration promoted the growth of Chlorella vulgaris,with the best aeration rate of 20%,while the excessive aeration inhibited the growth;the mathematical relationships between aeration ratio and ODmax,μmax and Cmax simulated by cubic curve equation were shown as follows:ODmax=170.63x3 -231.83x2 +84.341x+1.8439 (0〈x〈50%;R2 =0.9850),Cmax =15.844x3 -19.803x2 +6.8594x+0.0521 (0〈x〈50%;R2 =0.9285),μmax =8.1202x3 -11.428x2 +4.4963x+0.1173 (20%〈x〈30%;R2 =0.8581),the correlation coefficients were 0.985,0.9285,and 0.8581.Our research provides the theoretical basis for optimal cultivation and resource utilization of Chlorella vulgaris.