中文摘要：

背景 CCAAT/enhancer-binding 蛋白质(C/EBP ) 在 adipogenesis 期间为有丝分裂的同种细胞的扩大(MCE ) 被要求。C/EBPp 怎么在 3T3-L1 preadipocytes 的更早的区别节目调整 MCE，仍然是不清楚的。这份报纸的目的是理解 C/EBP 为什么为 preadipocyte 增长被要求，并且与染色质 immunoprecipitation (ChlP ) 识别 C/EBPP 的新目标基因 -on-chip。方法 Postconfluent 逮捕生长的 3T3-L1 preadipocytes 用一个标准区别协议被导致到区别。薄片与特定的 anti-C/EBP 抗体在正式就职以后在 20 个小时被执行。猛抛的 DNA 被放大，标记并且有老鼠倡导者 microarray 的 hybridized。与芯片实验与非特定的抗体在被执行的控制相比，当 110 候选人基因全部的候选人 genes.Results A 被识别，仅仅有增加超过 2 褶层的一个信号的基因被考虑。BTG3 联系了原子蛋白质(SMAR1， Banp ) 并且分成三部分包含主题 35 (Hls5， trim35 ) 是在涉及房间周期规定的 110 候选人基因之中的二目标基因；到 banp 和 trim35 的倡导者的 C/EBP3 的绑定被 ChlP-PCR 验证。结论 C/EBP 可以通过 banp 和 trim35 的激活调整 preadipocyte 增长。

英文摘要：

Background CCAAT/enhancer-binding protein β（C/EBPβ） is required for mitotic clonal expansion （MCE） during adipogenesis. It is still unclear how C/EBPp regulates MCE in the earlier differentiation programs of 3T3-L1 preadipocytes. The purpose of this paper was to understand why C/EBPβ is required for preadipocyte proliferation, and identify new target genes of C/EBPP with chromatin immunoprecipitation （ChlP）-on-chip. Methods Postconfluent growth-arrested 3T3-L1 preadipocytes were induced to differentiation using a standard differentiation protocol. Chip was performed at 20 hours after induction with specific anti-C/EBPβ antibodies. The precipitated DNA was amplified, labeled and hybridized with a mouse promoter microarray. Compared with the control in which the ChIP experiment was performed with non-specific antibody, only the genes with a signal increasing more than 2 fold were considered as candidate genes.Results A total of 110 candidate genes were identified. BTG3 associated nuclear protein （SMAR1, Banp） and tripartite motif-containing 35 （Hls5, trim35） were two target genes among the 110 candidate genes which are involved in cell cycle regulation; the binding of C/EBP3 to the promoter of banp and trim35 was verified by ChlP-PCR. Conclusion C/EBPβ may regulate preadipocyte proliferation through activation of banp and trim35.