中文摘要：

目的 观察复合羟丁基壳聚糖纳米载体携载CD133＋抗体温敏膜对人脐静脉内皮细胞（HUVEC）增殖、迁移及血液相容性的影响.方法 将HUVEC接种于裸钴铬合金片膜（裸金属支架组）、雷帕霉素药物涂层钴铬合金片膜（雷帕霉素支架组）、复合羟丁基壳聚糖纳米载体携载CD133＋抗体温敏膜钴铬合金片膜（抗温敏膜组）.培养24h后,在倒置相差显微镜下观察细胞形态;以MTT比色法检测细胞增殖活性,划痕试验检测细胞的迁移能力,ELISA检测各组细胞上清液中内皮素-1（ET-1）、降钙素基因相关肽（CGRP）、前列环素代谢物6-酮-前列腺素F1α（PGF1α）水平.结果 HUVEC在抗温敏膜上生长良好,形态正常.与裸金属支架组、雷帕霉素支架组相比,在抗温敏膜组存活的HUVEC数目增高（P均＜0.05）.HUVEC在抗温敏膜组上迁移率高于裸金属支架组、雷帕霉素支架组（P均＜0.05）.与裸金属支架组、雷帕霉素支架组相比,抗温敏膜组细胞分泌的ET-1水平降低;CGRP、PGF1α水平升高（P均＜0.05）.结论 复合羟丁基壳聚糖纳米载体携载CD133＋抗体温敏膜有助于HUVEC的增殖和迁移,对HUVEC的血液相容性良好.

英文摘要：

Objective To investigate the influence of hydroxybutyl-chitosan nanoparticles carries CD133＋ anti-thermosensitive membrane on the proliferation,migration and hemocompatibility of human umbilical vein endothelial cells （HUVECs）.Methods HUVECs were inoculated into bare cobalt-chromium alloy film （bare metal stent group）,sirolimus-eluting cobaltchromium alloy film （sirolimus stent group） and hydroxybutyl-chitosan nanoparticles carries CD133＋ anti-thermosensitive membrane cobalt-chromium alloy film （anti-thermosensitive membrane group）.After being cultured for 24 hours,the cell morphology was observed by inverted phase-contrast microscope.The cell proliferation was measured by MTT colorimeter.The ability of cell migration was detected by scratch tests.Enzyme linked immunosorbent assay （ELISA） was used to measure the levels of endothelin-1 （ET-1）,calcitonin gene-related peptide （CGRP） and prostacyclin metabolite 6-keto-prostaglandin F1α （PGF1 α） in all groups.Results HUVECs grew well in the anti-thermosensitive membrane group,with normal morphology.Compared withthe baremetal stent and sirolimus stent groups,the number of survived HUVECs in the anti-thermosensitive membrane group was higher （all P ＜0.05）.The migration ratio in the anti-thermosensitive membrane group was higher than that in the bare metal stent and sirolimus stent groups,respectively （all P ＜ 0.05）.Compared with bare metal stent and sirolimus stent groups,the concentration of ET-1 of anti-thermosensitive membrane group decreased,and increased in the CGRP and PGF1α levels （all P ＜0.05）.Conclusions Hydroxybutyl-chitosan nanoparticles carries CD133＋ anti-thermosensitive membrane cobalt-chromium alloy film can improvethe proliferation,migration and hemocompatibility of HUVECs.