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茶树荧光性绿斑病叶膜结构及相关生理变化研究
  • ISSN号:1000-369X
  • 期刊名称:《茶叶科学》
  • 时间:0
  • 分类:S571.1[农业科学—茶叶生产加工;农业科学—作物学] S432.2[农业科学—植物病理学;农业科学—农业昆虫与害虫防治;农业科学—植物保护]
  • 作者机构:[1]山东农业大学园艺科学与工程学院,山东泰安271018, [2]山东农业大学生命科学学院,山东泰安271018, [3]作物生物学国家重点实验室,山东寨安271018
  • 相关基金:国家自然科学基金(No.30571124)
中文摘要:

以福鼎大白为材料,通过钙过量处理诱导出茶树荧光性绿斑病,研究茶树荧光性绿斑病叶膜系统损伤、电渗率以及与膜透性有关的渗透调节物质和活性氧酶清除系统的变化。研究结果表明:茶树荧光性绿斑病叶的细胞质膜以及叶绿体、线粒体、内质网、液泡的膜系统均遭到不同程度的损伤,随着病害程度加重,丙二醛、电解质渗透率逐渐升高;与此同时可溶性糖、可溶性蛋白、脯氨酸等渗透调节物质含量也逐渐升高,超氧化物歧化酶、过氧化物酶活性在发病初期显著升高,后期降低,但活性均高于对照。以上结果说明:茶树荧光性绿斑病叶在发病期间虽然启动了活性氧清除的膜保护机制,但仍然无法逆转膜系统损伤的发生。

英文摘要:

In order to probe the membrane structure and its related physiological changes in the tea leaves with fluorescent green spot disease, the diseased tea leaves were picked from the Fu Ding Da Bai tea plant cultivated in the nutritional solution with excessive calcium, and cellular membrane system was observed by transmission electron microeope, the electrolytic leakage, the contents of osmosis and the antioxidative enzyme activities of diseased tea leaves were determined. The results showed that the plasma membrane, chloroplast membrane, mitochondrion membrane, endoplasmic reticulum membrane and vacuole membrane in diseased tea leaves were damaged in different degree. With the extending of stress and the disease, the malondialdehyde (MDA) content and the electrolytic leakage increased, meanwhile the soluble sugar content, the soluble protein content and the free proline content increased rapidly in the diseased tea leaves. The activity of superoxide dismutase (SOD), peroxidase (POD) increased significantly at the initial stage of the diseased tea leaves, subsequently decreased, but their activities were higher than normal tea leaves. On the basis of above results, although the antioxidative enzyme activities were increased, it was incapable of reversing the damage of membrane system of the diseased tea leaves.

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期刊信息
  • 《茶叶科学》
  • 中国科技核心期刊
  • 主管单位:中国科协
  • 主办单位:中国茶叶学会
  • 主编:朱永兴
  • 地址:浙江省杭州市梅灵南路9号
  • 邮编:310008
  • 邮箱:cykx@vip.163.com
  • 电话:0571-86651902
  • 国际标准刊号:ISSN:1000-369X
  • 国内统一刊号:ISSN:33-1115/S
  • 邮发代号:
  • 获奖情况:
  • 第五届全国农业期刊金犁奖,第五届全国农业期刊主编金犁奖,浙江省2005~2006年度优秀科技期刊1等奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国英国皇家化学学会文摘,英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:9506