中文摘要：

应用三维荧光光谱技术，研究了茶多酚溶液的三维荧光光谱特征，以及不同浓度、pH值、缓冲液、有机溶剂等因素对可见光区荧光的影响。研究表明：茶多酚溶液的三维光谱可分为三区（以激发波长／发射波长λ_ex／λ_em表示）：Ⅰ区：210nm／315nm、270nm／315nm；Ⅱ区：335nm／395nm；Ⅲ区：490nm／515nm。随浓度增大，茶多酚荧光的激发、发射波长逐渐红移。茶多酚浓度3mg／mL，溶液pH7．55条件下可灵敏检测Ⅲ区可见光区荧光；常用缓冲溶液对Ⅲ区荧光均有不同程度的增敏效果；质子化溶剂有利于Ⅲ区的荧光发射。据此可为茶多酚的快速、无损分析检测及应用开发提供新的思路。

英文摘要：

Three dimensional fluorescence spectral characteristics of tea polyphenols solution, and effects of different concentrations, pH values, buffers, organic solvents and other factors on the visible light region fluorescence were studied by using three dimensional fluorescence spectrometric technology. Results showed that, three dimensional fluorescence spectra of tea polyphenols in H20 could be divided into 3 regions（showed by λex/λem） ： Region Ⅰ ： 210 nm/315 nm, 270 nm/315 nm; Region Ⅱ ： 335 nm/395 nm; Region m ： 490 nm/515 nm. Excitation and emission wavelengths of the maximum fluorescence shifted towards the longer wavelength with the increase of tea polyphenols con- centration. Region Ⅲ fluorescence in visible light region can be sensitively detected at a concentration of 3 mg/mL and pH value of 7.55. Commonly used buffer solutions（ e. g. KH2PO4 -NaOH buffer, Na2 HPO4 - citric acid buffer, phosphate buffer and Tris - HC1 buffer） have certain degrees of sensitization effect to fluorescence intensity in Region Ⅲ. Polar protic solvents was beneficial for the fluorescence emission in Region Ⅲ. This study could provide new ideas and inspirations for fast, non-destructive analysis and developing application of tea polyphenols.