中文摘要：

应用高效液相色谱，对北方某水库5个采样点藻类细胞内3种微囊藻毒素（MC—LR，RR和YR）进行了为期一年的监测．研究了细胞内微囊藻毒素随时间的变化规律，并探讨了环境因子对细胞内微囊藻毒素产生的影响．结果表明，藻类细胞内3种微囊藻毒素被检出的时间不一致，LR异构体在4月份下旬即可检测到，而RR和YR异构体则要到5月份中旬才能检测到．LR和YR异构体在9月份出现高峰值，而RR异构体在10月份达到全年的最大值．4-9月份，LR异构体含量最大，RR异构体次之，YR异构体含量最小；10—12月份，RR异构体含量最大，10月和11月LR含量大于YR，但是12月份LR未检出，而YR含量比11月份还高．环境因子对细胞内微囊藻毒素的影响也不一致：溶解氧和硝酸盐氮都与3种异构体呈显著负相关；总磷、溶解性总磷和磷酸盐都与RR异构体呈显著正相关，氨氮和LR异构体之间存在显著负相关．

英文摘要：

The high performance liquid chromatography （HPLC） was used to monitor the intracellular microcystin - LR, - YR and - RR （MC - LR, - YR, - RR） within one year in five sampling cites： in a reservoir of northern China. The rule of the intracellular microcystins changing with time was explored, and the influence of environmental conditions on intracellular microcystins level was discussed. The MC - LR is detected until the month of April. The MC - YR content is below the limit of detection from February to April, and so is that of MC - RR from January to April. The peak contents of MC - YR and MC - LR occur in September, but MC - RR occur in October. From April to September, the MC contents order is ρ（ MC -LR） 〉ρ（ MC -RR） 〉ρ（ MC -YR）. The MC -RR content is highest among the three kinds of intracellular microcystins from October to December. The MC - LR content is higher than that of MC - RR in October and November. In December, the MC - LR content is below the limit of the detection, but the MC -YR is higher than that of November. The environmental factors have different influ- ence on intracellular microcystins level. Dissolved oxygen and nitrate are significant related factors to intracellular microcystins. Total phosphorus, dissolved total phosphorus and dissolved phosphorus correlate well with MC -RR. Ammonia nitrogen displays negative significantly correlation with MC -LR.