中文摘要：

利用简并引物PCR（polymerase chain reaction,PCR）方法和cDNA末端快速扩增（rapid amplification of cDNA ends,RACE）技术获得马氏珠母贝Pinctada martensii hsp90基因cDNA序列,全长为2 584 bp。根据所得序列设计定量特异性PCR引物,采用半定量RT-PCR以及实时荧光定量（real-time PCR）PCR检测了马氏珠母贝外套膜、鳃、肝胰腺、闭壳肌、性腺、腹足等组织中hsp90基因的表达水平。同时,利用荧光定量PCR技术检测了芘暴露处理前后马氏珠母贝肝胰腺组织中hsp90基因的表达水平。研究结果表明：马氏珠母贝hsp90基因在不同组织中的表达水平为性腺〉鳃〉肝胰腺〉外套膜〉腹足〉闭壳肌,表现出组织差异性。芘胁迫对马氏珠母贝hsp90基因的表达有一定的诱导作用,暴露后第1天和第5天,随染毒浓度的增加,hsp90基因的表达上调,呈现出一定的剂量-效应关系,于染毒后第7天基本恢复。研究结果显示,马氏珠母贝hsp90基因可以作为一种理想的分子生物标记物用于监测海洋环境中芘的污染。

英文摘要：

In this study, a heat shock protein 90 cDNA named hsp90 was cloned from pearl oyster Pinctada martensii by PCR（Polymerase Chain Reaction） with degenerate primers coupled with RACE（rapid amplification of cDNA ends）approaches. The full-length of hsp90 cDNA sequence in P. martensii was of 2 584 bp. According to the full-length cDNA sequence, hsp90 gene-specific PCR primers were designed. Hsp90 transcript could be detected in mantle、gill、hepatopancreas、adductor muscle、gonad、pleopod by the semi-quantitative RT-PCR and quantitative real-time RT-PCR analysis. At the same time, the effect of hsp90 gene expression levels from hepatopancreas in P. martensii after exposure to pyrene were evaluated using real-time quantitative-PCR. The results showed that hsp90 gene expression had tissues difference in P. martensii. The expression level ranking of hsp90 gene across tissues was gonads gill hepatopancreas mantle pleopod adductor muscle. The P. martensii hepatopancreas Hsp90 gene expression can be induced by pyrene after 1 d and 5 d exposure. The induction was exposure-dose dependent. The expression level of hsp90 recovered to normal level compared to the control level after 7 days exposure. As a result, hsp90 gene can be used as a molecular biomarker for monitoring pyrene pollution in the marine environment.