中文摘要：

H9c2细胞是来源于大鼠胚胎心脏组织的成肌细胞系,B组柯萨奇病毒（group B Coxsackievirus,CVB）是心肌炎和扩张型心肌病的主要病原。本研究观察了CVB3在H9c2细胞中的感染性,探讨H9c2细胞是否可用于CVB致心肌疾病的实验研究。用整合了增强型绿色荧光蛋白（EGFP）或海肾荧光素酶（RLuc）的CVB3重组株EGFP-CVB3、RLuc-CVB3攻击H9c2细胞及大、小鼠原代心肌细胞和骨骼肌细胞,应用荧光显微镜、流式细胞术和化学发光技术观察感染细胞的EGFP和RLuc表达,应用反转录-聚合酶链反应（RT-PCR）方法检测病毒在核酸水平的表达。结果显示,在病毒攻击的H9c2细胞未检测到EGFP和RLuc表达;RT-PCR显示在CVB3感染9 d后检测不到CVB3 RNA;CVB3毒株可感染小鼠心肌细胞、骨骼肌细胞和大鼠心肌细胞,但不感染大鼠骨骼肌细胞和H9c2细胞。本研究结果表明,H9c2细胞不是CVB3易感细胞,不能用于CVB3致心肌疾病的研究;该结果从病毒感染方面也支持H9c2具有大鼠骨骼肌细胞表型。

英文摘要：

H9c2 cells are a cardiac myoblast cell line derived from rat heart tissue.Group B Coxsackievirus（CVB） is the major pathogen of viral myocarditis and dilated cardiomyopathy.In this study,infectivity of CVB3 in H9c2 cells was observed to evaluate the value of H9c2 cells in the experimental study of CVB-induced myocardial diseases.H9c2 cells and cardiac and skeletal myocytes of BALB/c mouse and Sprague-Dawley（SD） rats were infected with two CVB3 variants integrated with enhanced green fluorescence protein（EGFP） or Renilla luciferase（RLuc）.The expressions of EGFP and RLuc were measured by fluorescence microscopy,flow cytometry,and chemiluminescence assays,and viral genomic RNA levels were detected by reverse transcriptase-polymerase chain reaction（RT-PCR）.Results showed that neither EGFP expression nor RLuc expression could be detected in H9c2 cells infected with EGFP-CVB3 or RLuc-CVB3.Viral RNA could not be detected by RT-PCR from day 9 post-infection.The cardiac and skeletal myocytes of BALB/c mouse and cardiac myocytes of SD rat could be infected by CVB3,but the skeletal myocytes of SD rat and H9c2 cells could not.Taken together,this study suggests that H9c2 cell is not a permissive cell for CVB3,and is not suitable for the study of CVB-induced myocardial diseases.The results also support the notion that H9c2 cells exhibit properties of skeletal myocytes from the view of infection.