中文摘要：

发生在蛋白质离氨酸残余的 translational 以后修正(PTM ) ，或蛋白质离氨酸修正(PLM ) ，在调整生物过程起关键作用。由于 PLM 底层和新奇 PLM 类型的发现的数量的爆炸扩大，这里，我们极大地更新了我们的以前的研究，并且介绍了蛋白质离氨酸修正数据库(PLMD ) 的一个更综合的资源。在 PLMD ，我们完全收集了，包括 ubiquitination 并且综合在越过为 PLM 的多达 20 种类型的 176 优核质和初核质的 53,501 蛋白质的 284,780 个修正事件， acetylation ， sumoylation ， methylation ， succinylation ， malonylation ， glutarylation ， glycation ， formylation ， hydroxylation ， butyrylation ， propionylation ， crotonylation ， pupylation ， neddylation ， 2-hydroxyisobutyrylation ， phosphoglycerylation ， carboxylation ， lipoylation 和 biotinylation 。用数据集合，基于主题的分析为每种 PLM 类型被执行，并且结果证明不同 PLM 类型优先地为修正认出不同顺序主题。而且，各种各样的 PLM synergistically 与对方一起由相互的串音安排特定的细胞的生物过程，并且我们完全发现 65,297 在一样的离氨酸残余上涉及 PLM 同现的 90 种类型的 PLM 事件。最后，各种各样的选择被提供存取数据，当原来的参考书和另外的注解也是为每 PLM 底层的现在时。一起拿，我们预计 PLMD 数据库能为 PLM 的进一步的研究用作一个有用资源。PLMD 3.0 在 PHP + MySQL 并且自由地被实现在 http://plmd.biocuckoo.org 可得到。

英文摘要：

Post-translational modifications（PTMs） occurring at protein lysine residues,or protein lysine modifications（PLMs）,play critical roles in regulating biological processes.Due to the explosive expansion of the amount of PLM substrates and the discovery of novel PLM types,here we greatly updated our previous studies,and presented a much more integrative resource of protein lysine modification database（PLMD）.In PLMD,we totally collected and integrated 284,780 modification events in 53,501 proteins across 176 eukaryotes and prokaryotes for up to 20 types of PLMs,including ubiquitination, acetylation, sumoylation, methylation ,succinylation,malonylation,glutarylation,giycation,formylation,hydroxylation,butyrylation,propionylation,crotonylation,pupylation,neddylation,2-hydroxyisobutyrylation,phosphoglycerylation,carboxylation,lipoylation and biotinylation.Using the data set,a motif-based analysis was performed for each PLM type,and the results demonstrated that different PLM types preferentially recognize distinct sequence motifs for the modifications.Moreover,various PLMs synergistically orchestrate specific cellular biological processes by mutual crosstalks with each other,and we totally found 65,297 PLM events involved in 90 types of PLM co-occurrences on the same lysine residues.Finally,various options were provided for accessing the data,while original references and other annotations were also present for each PLM substrate.Taken together,we anticipated the PLMD database can serve as a useful resource for further researches of PLMs.PLMD 3.0 was implemented in PHP ＋ MySQL and freely available at http：//plmd.biocuckoo.org.