中文摘要：

建立了间接竞争酶联免疫吸附法测定血浆中芍药苷浓度：存10～200μg/L浓度范围内，线性关系良好，线性回归方程为y=-0．1721logC＋1．9645，相关系数0．9927；回收率为89％～102％。运用本方法测定了大鼠一次灌胃后血浆中的芍药苷浓度。比较了分别给药芍药甘单体及中药复方四逆散提取物后，血浆中的芍药苷浓度。研究结果表明：四逆散配伍组方后，芍药苷的生物利用度较低。

英文摘要：

A method was developed for the determination of paeoniflorin in plasma by competitive enzyme- linked immunosorbent assay（ELISA）. The artifical antigen was used as immunogen and applied in New Zeal- and rabbits to get the anti-paeoniflorin antibodies. Hapten coupled with OVA was used as coating antigen. The linear concentrations ranged from 10 μg/L to 200 μg/L and the calibration curve was Y = -0. 1721 logC ＋ 1. 9645. Recoveries for the standard addition paeoniflorin to blank palsma were 89% - 102%. The method has been successfully applied to determine the concentration of paeoniflorin in rat plasma. After an oral administra- tion of the Si-Ni-San decoction, the bioavailability of paeoniflorin was lower than a single dose of paeoniflorin.