中文摘要：

目的探讨光动力学治疗（PDT）后的肿瘤细胞裂解物对卵巢上皮性癌（卵巢癌）大鼠的抗肿瘤作用。方法选择6～8周龄Fischer 344雌性大鼠，实验分为PDT组（腹腔注射PDT后的肿瘤细胞裂解物）、冻融组（腹腔注射冻融后的肿瘤细胞裂解物）、盐水组（腹腔注射生理盐水），间隔1周，3组均腹腔注射大鼠卵巢癌细胞系NuTu19细胞10^5个；设仅腹腔注射生理盐水者为空白对照组。采用酶联免疫斑点（ELISPOT）实验定量检测经PTD后的肿瘤细胞裂解物和冻融后的肿瘤细胞裂解物刺激后各组大鼠脾淋巴细胞中分泌肿瘤特异性γ干扰素（IFN-γ）的细胞数；乳酸脱氢酶（LDH）释放试验检测各组大鼠脾淋巴细胞中细胞毒T淋巴细胞（CTL）的杀伤活性；观察各组大鼠的生存时间以了解肿瘤细胞裂解物的抗肿瘤效果。结果经PDT后的肿瘤细胞裂解物刺激后，PDT、冻融、盐水、空白对照组大鼠脾淋巴细胞中分泌肿瘤特异性IFN-γ的细胞数分别为（448．8±34．2）、（211．2±47．9）、（43．3±11．1）、（16．1±2．4）个，PDT组细胞数最多，分别与其他3组比较，差异均有统计学意义（P均〈0．05）；经冻融后的肿瘤细胞裂解物刺激后，上述4组大鼠脾淋巴细胞中分泌肿瘤特异性IFN-γ的细胞数分别为（151．7±22．6）、（188．7±53．0）、（18．2±12．2）、（8．8±7．7）个，PDT组与冻融组比较，差异无统计学意义（P〉0．05）。PDT组脾淋巴细胞中CTL的杀伤活性较其他3组明显增强（P〈0．05）。PDT、冻融、盐水组大鼠中位生存时间分别为234、171、168d，空白对照组观察至截止时间全部存活，PDT组大鼠中位生存时间长于冻融和盐水组，差异有统计学意义（P〈0．05）。结论PDT后的肿瘤细胞裂解物在大鼠体内诱发了特异性的抗肿瘤免疫反应，并可延长卵巢癌大鼠的生存时间，具有较好的抗肿瘤作?

英文摘要：

Objective To study the antitumor effects of photodynamic therapy （PDT）treated by ovarian cancer cell lysates in rat epithelial ovarian cancer in vivo. Methods Female Fischer344 rats of 6 - 8 weeks were allocated to four groups （ n = 8 each） ： PDT group （ inoculated intraperitoneal with PDT tumor cell lysates）, freeze/thaw group （inoculated intraperitonealy with freeze-thaw tumor cell lysates）, normal saline group （inoculated intraperitoneal with normal saline） and control group. Rat epithelial ovarian cancer NuTul 9 cells were injected into all rats by intraperitoneal at day 7, while injected with normal saline in control group. The number of tumor specific interferon-γ （IFN-γ） secreting splenocytes was quantified by enzyme linked immunospot（ELISPOT） assay, the eytotoxic T lymphocyte（CTL） activity of splenoeytes was measured by lactate dehydrogenase （LDH） analysis and tumor growth and the survival time of rats were also observed. Results Stimulated by PDT tumor cell lysates, the number of tumor specific IFN-γ secreting splenocytes in PDT group, freeze/thaw group, normal saline group and control group were 448.8 ± 34. 2, 211.2 ± 47. 9,43.3 ± 11.1,16. 1 ± 2.4 respectively, which were significant differences among of them（P 〈 0. 05）. Stimulated by freeze/thaw tumor cell lysates, the number of tumor specific IFN-γ secreting splenocytes in four groups were 151.7 ± 22. 6,188. 7 ± 53.0, 18. 2 ± 12. 2,8. 8 ± 7. 7 respectively, which were not significant differences among of them （ P 〉 0. 05 ） . Cytotoxicity of splenocytes of PDT group increased significantly than that in other three groups（ P 〈 0. 05 ）. Except rats in control group were all alive until the experiment ended, the mean survival time of other rats were 234 d in PDT group, 171 d in freeze/ thaw group and 168 d in normal saline group, which in PDT group was significantly higher than those in freeze/thaw group and normal saline group（P 〈 0. 05）. Conclusions Rats treated by PDT tumor cell