中文摘要：

目的制备具有良好生物相容性的磁性纳米粒，并对其光热性能、细胞毒性及体外光热治疗效果进行研究。方法采用水热法合成Fe304，分别利用羧甲基壳聚糖（CMCTS）及壳聚糖（chitosan）对其进行功能化修饰，采用TEM、TGA等手段对制备的载体进行表征，考察纳米粒的稳定性及光热性能，同时采用MTr方法研究载体的细胞毒性、评价其光热治疗效果。结果所制备的Fe304粒径均一、分散良好，CMCTS修饰的Fe30。具有良好的稳定性；Fe304-CMCTS纳米粒无明显毒性，Fe，04-Chi-tosan由于稳定性原因显示一定的细胞毒性；光热治疗结果显示，浓度为60μg·mL“的2种载体在808nm激光（2W·cm^-1）照射5min时，可杀伤90％以上的细胞。结论所制备的磁性Fe3O4-CMCTS纳米粒具有良好的稳定性、较低的细胞毒性及优良的光热效果，可以作为新型光热材料深入研究。

英文摘要：

OBJECTIVE To prepare magnetic nanoparticles with good biocompatiblity and evaluate the photothermal effect, cyto- toxicity and photothermal abltion of cancer cells in vitro, METHODS The magnetic nanoparticles were sythesized using a modified solvothermal reaction and then modified with O-carboxymethyl chitosan （CMCTS） and chitosan respectively. The prepared magneic particles were characterized with TEM and TGA. Then the stability and photothermal effect were evaluated. MTY method was ued to study the cytotoxicity and cancer therapeutic effect of the magnetic nanoparticles. RESULTS The obtained Fe3 04 nanoparticles were uniform and well dispersed. After modification with CMCTS, Fe3 04 showed good stability and photothermal effect. No obvious cytotoxic- ity was observed in Fe3 O4-CMCTS treated cells. Fe3 04-chitosan exhibited some cytotoxicity for the poor stability. After being treated with Fes 04 -CMCTS or Fe3 04-Chitosan at the concentration of 60 μg · mL^-1 and exposed to 808 nm laser for 5 min at a low power den- sity of 2 W · cm^-2, over 90% of KB cells were destructed. CONCLUSION The obtained Fe3 04-CMCTS magnetic nanoparticles had favourable stability, low cytotoxicity and excellent photothermal effect, and should be studied further as a novel photothermal agent.