中文摘要：

目的 探讨核转录因子κB（NF—κB）及其诱导的细胞因子在大鼠角膜炎中的表达及二硫代氨基甲酸吡咯烷（PDTC）对其表达的影响。方法 选择112只大鼠建立角膜炎模型，按随机数字表法分为炎性组56只和PDTC预处理组56只。模型制作前30min，大鼠球结膜下分别注射生理盐水（炎性组）和PDTC（PDTC预处理组），每组按脂多糖（LPS）刺激后不同时间又分为0．5、1．0、3．0、6．0、12．0、24．0及72．0h亚组，每亚组8只鼠。裂隙灯显微镜下观察大鼠的眼部变化；病理组织切片观察角膜形态学改变；免疫组织化学染色检测角膜NF—κB p65的表达；逆转录聚合酶链反应（RT—PCR）检测角膜肿瘤坏死因子α（TNF—α）mRNA的表达。结果 LPS刺激后炎性组大鼠角膜组织明显水肿，大量炎性细胞浸润，胶原纤维排列紊乱；免疫组织化学染色显示LPS刺激后0．5h即可见NF—κB阳性细胞，并表达逐渐增强，3．0～12．0h最强，0．5～24．0h间均较PDTC预处理组明显增多（P〈0．01）；TNF—αmRNA的表达在LPS刺激后0．5h就开始升高，3．0～12．0h最强，0．5～24．0h间均较PDTC预处理组明显增高（P〈0．01）。结论 NF—κB及其诱导的TNF—α在角膜炎中发挥重要作用，PDTC可能通过抑制NF—κB的活性减轻角膜损伤。（中华眼科杂志，2006，42：699—703）

英文摘要：

Objective To investigate the expression of nuclear factor-kappa B （NF-κB） and cytokines in lipopolysaccharide （LPS）-induced keratitis of rats, and the effect of pyrrolidine dithiocarbamate （PDTC） on its expression. Methods A LPS-induced keratitis model was established in Wistar rats. Thirty minutes before LPS exposure, PDTC and normal saline were injected into subconjunctival tissues separately. At 0. 5, 1.0, 3.0, 6. 0, 12.0, 24. 0 and 72.0 h after LPS exposure, the rats were examined with slit-lamp microscope. Then they were sacrificed and the corneas were excised for routine histological analysis. Immunohistochemical staining with an antibody against activated NF-κB was performed to detect the expression of NF-κB. The change of TNF-α mRNA expression was identified by reverse transcriptase polymerase chain reaction （RT-PCR）. Results Histology findings demonstrated that corneas exposed to LPS showed significant changes in corneal structure, edema and pronounced inflammatory cells infiltration were observed. Both symptoms and damages of the cornea were lesser in the rats of PDTC group than that of the control keratitis group. Compared with PDTC group, the activation of NF-κB and the expression of TNF-α mRNA were significantly upregulated after LPS challenge 0. 5-24.0 h （P 〈 0.01 ）, separately; peak expression of NF-κB and TNF-α mRNA were observed at 3.0-12. 0 h after LPS exposure. Conclusions The expression of NF-κB and TNF-α mRNA induced by NF-κB plays an important role in the pathogenesis of keratitis. The inhibitor of NF - κB , PDTC , can relieve the cornea from damages produced by keratitis. （Chin J Ophthalraol, 2006,42：699-703 ）