中文摘要：

目的应用超高场强MR T2~＊-mapping序列定量评估实验性自身免疫性脑脊髓炎（EAE）动物模型的脊髓病变。方法 6~8周雌性C57BL/6小鼠,MOG35-55诱导制备EAE模型10只（实验组）,同龄健康对照组10只。在造模后第18天,分别对实验组和对照组脊髓行T2~＊-mapping序列扫描,经后处理软件分析,比较实验组和对照组脊髓感兴趣区T2~＊值的差异。结果实验组第12天大鼠的体质量开始减轻,第14天神经学评分逐步增加。对照组体质量无明显减轻。MR T2~＊加权像显示实验组脊髓内病灶呈不规则点片状稍高信号;对照组脊髓呈均匀等信号。T2~＊值实验组[（12.66±1.78）ms]和对照组[（17.80±2.06）ms]差异有统计学意义（P〈0.001）,实验组各评分T2~＊值之间差异无统计学意义（P〉0.05）。结论超高场强MR T2~＊-mapping序列能够清晰显示小鼠脊髓病灶,并且可对病灶组织内铁含量进行定量测定,为EAE模型脊髓病变的无创检查提供新方法。

英文摘要：

Objective To quantitative evaluate of pathological changes of spinal cord in experimental autoimmune encephalitis（EAE）using high-field 7.0Tanimal MRI T2＊-mapping.Methods Female C57BL/6mice of 6—8weeks old were induced by emulsifying MOG35-55 to make 10 models of EAE（EAE group）.Meanwhile,10 healthy mice were prepared as control group.In the 18 days,T2~＊-mapping on the lumbar spinal cord of EAE group and control group were performed.After postprocessing analysis,the differences of T2~＊values of EAE group and control group were compared.ResultsWeight of EAE group started to reduce from the 12~（th） day and the neurological score started to gradually increase from the 14~（th） day;The control group was in good condition and without weight loss.MRI T2~＊phase diagram showed hyperintense signal around the corresponding lumbar lesion of mice in EAE group.However,the spinal cord showed homogeneous signal intensity in control group.The difference of T2~＊values had significant statistiacal between EAE group and control group（[12.66±1.78]ms vs[17.80±2.06]ms,P〈0.001）.There was no significant difference among different neurological score mice in EAE model（P〉0.05）.Conclusion High field MRI T2~＊-mapping sequence can clearly show the spinal cord lesions in EAE mice and it can be a tool for quantitative determination of the iron in the lesion.It is a new method for the noninvasive examination of spinal cord lesions in EAE model.