中文摘要：

目的：研究经不同途径接种乙脑病毒prME蛋白编码基因DNA疫苗所诱导的细胞免疫。方法：将乙脑病毒prME蛋白编码基因DNA疫苗（命名为pJME）分别以肌注、滴鼻两种免疫方式免疫BALB/c鼠,流式细胞仪检测脾脏CD4＋/CD8＋T淋巴细胞亚群,乳酸脱氢酶释放实验测定脾脏特异性细胞毒性T淋巴细胞（CTL）活性。结果：pJME肌注组与滴鼻组免疫鼠CD4＋T淋巴细胞百分比（30.91＋0.72）%、（27.89＋0.42）%,均高于灭活疫苗与载体肌注组（23.73＋0.17）%、（23.78＋0.21）%（P〈0.05）,肌注组显著高于滴鼻组（P〈0.05）;而CD8＋T淋巴细胞百分比（14.62＋0.25）%、（13.73＋0.50）%,也均高于灭活疫苗和载体肌注组（10.80＋0.39）%、（11.97＋0.06）%（P〈0.05）,肌注组与滴鼻组无显著差异（P〉0.05）;两组有明显CTL效应（pJME肌注组29.1%,pJME滴鼻组17.1%）,明显高于载体肌注组6.0%,pJME肌注组明显优于滴鼻组。结论：pJME以两种途径免疫鼠时可诱导明显细胞免疫反应。

英文摘要：

Objective：To study cellular immunity induced by DNA vaccine encoding prME protein derived from Japanese encephalitis virus in the routes of different inoculation.Methods：BALB/c mice were vaccinated with DNA vaccine,named pJME,encoding Japanese Encephalitis virus prME protein via intramuscular and intranasal injection respectively.CD4＋/CD8＋ T lymphocyte subsets of mouse spleen cells were assayed with flow cytometry technique,and CTL activity was detected with lactic dehydrogenase release test method.Results：Both of CD4＋ T lymphocyte percentage [intramuscular：（30.91＋0.72）%,intranasal：（27.89＋0.42）%] produced by pJME via intramuscular and intranasal injection respectively were higher than that by inactivated vaccine and vector.[inactivated vaccine：（23.73＋0.17）%,vector：（23.78＋0.21）%]（P 〈 0.05）,however,pJME group via intramuscular injection was still higher than that via intranasal injection（P 〈 0.05）.Both of CD8＋ T lymphocyte percentage [intramuscular：（14.62＋0.25）%,intranasal：（13.73＋0.50）%]produced pJME via intramuscular and intranasal injection respectively were also higher than that by inactivated vaccine and vector[inactivated vaccine：（10.80＋0.39）%,vector：（11.97＋0.06）%]（P 〈 0.05）,but there was no significant difference（P 〉 0.05） between pJME group via intramuscular injection and that via intranasal injection.Both of above groups could promote CTL activity（pJME intramuscular：29.1%,pJME intranasal：17.1%）.pJME group via intramuscular injection was higher than that via intranasal injection.Conclusion：pJME via intramuscular and intranasal injection respectively can induce obvious cellular immunity.