中文摘要：

目的：采用WST-8检测培养小鼠脾NK细胞增殖活性，并探讨其杀伤活性。方法：采用WST-8检测体外培养的不同细胞数量的小鼠脾NK细胞增殖活性，并采用乳酸脱氢酶法检测其杀伤活性。结果：小鼠脾NK细胞在培养第3～5d增殖最旺盛，第5d进入平台期。1×10^6孔的细胞数检测较敏感。培养5d的NK细胞杀伤活性随着效靶细胞比例的增加而增加，在效靶比为20：1时，杀伤活性最大。结论：WST-8是一种理想的检测小鼠脾NK细胞增殖活性的方法，但其对NK细胞的敏感性较低，培养的NK细胞在效靶比为20：1时杀伤活性最大。

英文摘要：

Objective：To detect the proliferation of natural killer（NK） cells from spleen of mouse with WST - 8 and investigate their killing activity. Methods： The proliferation of different cell population of NK cells from mouse＇s spleen cultured with IL - 2 was examined with WST - 8 and the killing activity of these NK cells was examined with lactic acid dehydrogenase. Results： The increased logarithmic phase of these cultured NK cells appeared in 3-5 days and the platform did in the fifth day. The content of 1 × 106 cell every bore was more sensitive to the examination than the other contents. The killing activity of the NK cells increased as the ratio of effector to target cells increased. When the ratio of effector to target cells was 20 ： 1, the killing activity of the NK cells was the greatest. Conclusion： WST - 8 is a good way for the detection of proliferation of NK cells of mouse＇s spleen although it is not too sensitive to NK cells. When the ratio of effector to target cells was 20 ： 1, the killing activity of the NK cells was the maximal.