中文摘要：

目的研究一种简单、高灵敏、高选择性检测溶菌酶的比色方法。方法根据比色法进行定性分析，利用紫外可见分光光度法，根据610nm和525nm波长下吸光度之比（A610／A525）进行溶菌酶的定量检测。结果A610／A525药与溶菌酶浓度在10—100nmoVL之间呈良好的线性关系，线性方程为A610／h525=0．0073c（nmol/L）＋0．254（r2=0．9945），检出限为3．4nmoWL（S／N=3）。结论所建立的检测溶菌酶的比色方法简单、选择性好、灵敏度高，可作为一种适体传感器的模型。

英文摘要：

lysozyme. Aim A simple, highly specific and sensitive colorimetric method is developed for the determination of Methods UV-vis spectroscopy and colorimetric method. Results It is found that the ratio of absorption peaks of the aptamer-AuNPs at 610 and 525 nm （A610/A525 ） was proportional to the concentration of lysozyme in the range from 10 nM to 100 nM. The linear regression fitting is A610/A525 =0. 007 3C （nmol/L） ＋0. 254 （r2 =0. 994 5 ） and the detection limit of lysozyme was about 3.4nM （ S/N = 3 ）. This method has also shown high selectivity for lysozyme over thrombin, hIgG and BSA. Conclusion It demonstrates that anti-lysozyme aptamer and AuNPs based calorimetric method is a promising method for simple, highly sensitive and selective determination of special proteins.