中文摘要：

在前期构建辽宁绒山羊皮肤cDNA文库的基础上，测通得到了新基因UMP／CMP激酶的全长序列，并对UMP／CMP激酶基因进行生物信息学分析。然后，通过RT—PCR检测该基因在心、肝、脾、肺及肾中的表达。生物信息学分析显示，UMP／CMP激酶基因全长1081bp，能够编码含有228个氨基酸的蛋白质，亮氨酸、甘氨酸、赖氨酸含量较高。UMP／CMP激酶含有较高比例的无规卷曲结构（51．75％），并发现其含有UMP／CMP激酶蛋白结构域。蛋白三级结构、进化树分析显示，辽宁绒山羊和牛的UMP／CMP激酶基因的同源性最高。UMP／CMP激酶基因存在跨膜结构域，并且存在信号肽剪切位点。该基因中有8个Ser，2个Thr，2个Tvr可能成为蛋白激酶磷酸化位点。RT—PCR检测显示，UMP／CMP激酶基因特异地表达于皮肤。这些研究结果为深入研究辽宁绒山羊的种质遗传特性奠定了基础。

英文摘要：

In order to study the genetic characteristics of Liaoning newbreed cashmere goat,on the basis of construction of a skin cDNA library and isolation of a full-length cDNA clone termed UMP/CMPK,the bioinformatics analysis and semi- quantitative RT-PCR were used to detect the expression of the UMP/CMPK gene in all internal organs. A bioinformatics analysis showed that the UMP/CMPK gene encoded a putative 228 amino acid protein that exhibited a high content of leucine, glycine and lysine and had a lower content of histidine and tryptophan. The secondary structure prediction revealed a high proportion of random coils （51.75%）. UMP/CMPK contained a conservative UMP/CMP kinase protein structure domain. UMP/CMPK of the goat and cattle had the highest homology. UMP/CMPK also had transmembrane domain and signal peptide. 8 Ser,2 Thr and 2 Tyr may be phosphorylation sites in CMP/UMPK protein.RT-PCR analysis showed that UMP/ CMPK were only present in goat skin. This research provides an experimental basis for the further study of the genetic characteristics of the goat.