中文摘要：

目的通过观察低氧致大鼠肺动脉平滑肌细胞丝氨酸／苏氨酸蛋白激酶2蛋白表达水平变化与低氧致肺动脉平滑肌细胞增殖的关系，探讨丝氨酸／苏氨酸蛋白激酶2在低氧肺血管重建中的调控作用。方法组织块法培养肺动脉平滑肌细胞，采用蛋白印迹技术检测蛋白表达水平，采用甲基噻唑基四唑法、氚-胸腺嘧啶核苷掺入法检测肺动脉平滑肌细胞增殖。结果低氧刺激肺动脉平滑肌细胞不断增殖，常氧下氚-胸腺嘧啶核苷掺入检测值为0．372±0．059，随着低氧处理时间的延长氚-胸腺嘧啶核苷掺入检测值不断升高，其中低氧12h达到0．703±0．100，与常氧组比较差异显著：常氧下甲基噻唑基四唑检测值为8374．39±545．31，随着低氧处理时间的延长，甲基噻唑基四唑检测值不断升高，低氧24h达到11208．35±678．82，与常氧组比较差异显著。各组均检测出丝氨酸／苏氨酸蛋白激酶2蚕白的表达，图像定量分析显示各组蛋白表达与细胞增殖改变密切相关。结论丝氨酸／苏氨酸蛋白激酶2信号通路可能在低氧肺动脉高压中调节肺动脉平滑肌细胞增殖。

英文摘要：

Aim To observe the changes of serine/threonine kinase 2（Akt 2）protein expression in pulmonary arteriy smooth muscle cells （PASMC） induced by hypoxia in rats, and to investigate the value of Akt 2 signaling pathway in hypoxia pulmonary hypertension （HPH）. Methods The pure PASMC was cultured by tissue-sticking methods. Akt 2 protein expressions were determined by Westem blotting after PASMC were exposed to hypoxia for 2 h, 8 h, 12 h and 24 h respectively. The changes of PASMC proliferation were determined by M＇IT and 3H-TdR incorporated way. Results The value of 3H-TdR was 0. 372 ±0. 059 under norrnoxia. And accompanying the prolongation of hypoxia time, the value of 3H-Td-Pt kept heightening. It was 0.703 ± 0. 100 in 12 h and significantly different from normoxia group; The value of MTT was 8374.39 ± 545.31 under normoxia. And accompanying the prolongation of hypoxia time, the value of MTT kept heightening. It was 11208.35 V 678.82 in 24 h group and significantly different from nomloxia group. The protein of Akt 2 could be detected in all groups, and the expressive level of protein showed a possible relationship with PASMC prolferation. Conclusion The results suggest that Akt 2 signaling pathway may play an important role in the hypoxia pulmonary hypertension.