中文摘要：

目的：观察六味地黄汤对单侧输尿管梗阻（UUO）大鼠模型中单核趋化蛋白-1（MCP-1）及转化生长因子-β1（TGF-β1）表达的影响。方法：将72只雄性SD大鼠随机分为模型组、依那普利组、六味地黄汤组、假手术组,行UUO术后即预药物干预。于术后第7、14、21天分别处死各组大鼠。用HE和Masson染色法观察各组大鼠术后不同时间点肾脏病理改变,用免疫组化法检测肾小管间质MCP-1及TGF-β1表达。结果：与假手术组比较,其余3组各时间点MCP-1及TGF-β1表达均明显高于假手术组（P〈0.05）;六味地黄汤组的MCP-1和TGF-β1表达明显低于模型组各时间点（P〈0.05）,高于依那普利组（P〈0.05）。结论：六味地黄汤可下调MCP-1和TGF-β1的表达,减缓UUO大鼠肾小管间质损害,可作为梗阻性肾病辅助用药。

英文摘要：

Objective：To observe the effect of Liuweidihuang decoction（LD）on expression of monocyte chemotactie protein （MCP- 1 ） and transforming growth factor（TGF - β1） in unilateral ureter obstraction（UUO） rats. Methods： Seventy - two male Sprague - Dawley rats were randomly divided into four groups： the model group, the sham - operation group, the Enalapril group and LD group. The kidneys of rats were harversted at 7, 14 and 21 d after the operation. Immunohistochemistry was used on the renal tissue for MCP- 1 and TGF - β1 after7,14 and 21 day. Histological changes were also observed by HE and Masson staining. Results： Compared with those in the sham - operation group, the levels of MCP - 1 and TGF -β1 expression was obviously higher than those in the other groups on day 7,14,and 21（P〈0.05）.The MCP- 1 and TGF-β1 of in LD group was higher than that in Enalapril group on day 7,14, and 21 （ P 〈 0.05）. Conclusion： LD can decrease the expression of the UUO MCP - 1 and level and TGF -β1 and reduce tubuloninterstitial injury, and LD can be an axillary drug for obstruct kidney disease.