中文摘要：

目的：筛选转基因何首乌毛状根的最优培养基，并考察诱导子对其生长的影响。方法：绘制转基因何首乌毛状根在MS、1／2MS、B5和White4种不同培养基中的生长曲线，考察不同培养基对其生长的影响；考察以MS作为培养基时，2种常见的诱导子水杨酸、茉莉酮酸甲酯对毛状根累积的影响。结果：从整个生长周期来看，毛状根在MS培养基中的生长曲线高于其他3个；不同浓度的水杨酸、茉莉酮酸甲酯对转基因何首乌毛状根的生长有抑制作用。结论：转基因何首乌毛状根在高浓度盐的MS培养基中长势最优，2种诱导子对毛状根的累积均有抑制作用。

英文摘要：

Objective：To optimize the culture medium of transgenic Polygonium multiflorum hairy root and the effect of different elicitors on its growth. Methods：Measured the growth curve of hairy root in four media, including MS, 1/2 MS, B5 and White and esti- mated the effects of elicitors on the hairy root biomass. Results ： MS medium was the most suitable of the four media for hairy root growth. The different concentrations of elicitors, salicylic acid and methyl jasmonate, inhibited the biomass accumulation of hairy root. Conclusion：MS is the optimum medium for transgenic Polygonium multiflorum hairy root. Two usual plant elicitors, salicylic acid and methyl jasmonate, can inhibit the growth of transgenic hairy root.