中文摘要：

以产酸克雷伯氏杆菌（Klebsiella oxytoca）ME-UD-3为出发菌株，经紫外线及硫酸二乙酯复合诱变后分别在葡萄糖浓度逐渐提高的液体培养基中进行富集培养，筛选获得了一株耐高糖的2，3-丁二醇高产突变菌株KoxytocaME-UD-3-4；该菌株的初始葡萄糖耐受浓度从出发菌株的120g／L提高到300g／L以上，在初始葡萄糖浓度为95g／L的条件下发酵培养，与出发菌株相比发酵时间缩短了8h，2，3．丁二醇的产量由原来的38．5g／L提高到43．0g／L，生产强度从0．80g／L·h提高到1．08g／L·h，转化率达到了理论值的91％。

英文摘要：

Klebsiella oxytoca ME-UD-3-4 with high glucose tolerance was obtained from the original strain ME-UD-3 after mutagenesis by UV coupled with diethyl sulfate and following continous subculture in the broth with gradually increased glucose concentration. Compared with the original strain which could only endure 120g/L glucose, the mutant was able to tolerate as high as 300g/L glucose. 95g/L glucose was depleted by the mutant in 40h,which was 8h shorter than that of the original strain; and the final 2,3-butanediol concentration and productivity of which achieved to 43.0g/L and 1.08g/L · h, respectively, with the yield up to 91% of the theoretical yield.