中文摘要：

目的探讨P2X7受体在HIV-1包膜糖蛋白gp120所致BV2小胶质细胞损伤中的作用,以及柚皮苷通过作用于P2X7受体对gp120所致BV2小胶质细胞损伤产生的保护作用。方法通过gp120处理BV2小胶质细胞建立细胞损伤模型,并通过MTS比色法检测细胞损伤程度和柚皮苷是否对损伤细胞具有保护作用;应用逆转录PCR（RT-PCR）及蛋白质印迹法（Western blot）检测P2X7受体的表达变化。结果 gp120处理24h后,与对照组比较,gp120组细胞存活率显著下降（P〈0.01）;gp120＋柚皮苷组的细胞存活率与gp120组比较有所上升（P〈0.01）,但与gp120＋BBG组比较差异无统计学意义（P〉0.05）;RT-PCR及Western blot的检测结果显示,gp120组小胶质细胞的P2X7受体m RNA及蛋白均比对照组明显升高（P〈0.01）,而gp120＋柚皮苷组与gp120组比较有所下降（P〈0.01）。结论 P2X7受体参与gp120所致BV2小胶质细胞损伤,柚皮苷可能通过抑制P2X7受体的表达上调对gp120所致细胞损伤产生保护作用。

英文摘要：

Objective To explore the effect of P2X7 receptor on HIV-1 envelope glycoprotein gp120（gp120）-induced injury in BV2 microglial cells, and research the protective effect of naringin on gp120-induced injury mediated by P2X7 receptor in BV2 microglial cells. Methods BV2 microglial cell injury model was established by gp120 treatment; The damage degree of BV2 microglial cells and the protective effect of naringin were measured by MTS colorimetry. The expression changes of P2X7 receptors were detected by RT-PCR and Western blot. Results Compared with Ctrl group, the cell survival rate of gp120 group decreased significantly after 24-h treatment（P〈0.01）. The cell survival rate of gp120 ＋ naringin group increased compared with gp120 group（P〈0.01）, while there was no difference compared with gp120 ＋ BBG group（P 〉0.05）. The results of RT-PCR and Western blot showed that the expression of P2X7 m RNA and protein in gp120 group was increased significantly compared with Ctrl group（P〈0.01）, and it was decreased in gp120 ＋ naringin group compared with gp120 group（P〈0.01）. Conclusions P2X7 receptor involved in gp120-induced injury in BV2 microglial cells, and naringin may play a protective effect on gp120-induced injury by inhibiting the up-regulation of P2X7 receptor expression.