奴卡氏菌属, Rhodococcus 和 Streptomyces,放射菌类家庭的所有成员,是有满意、能形成菌丝体的高 G+C 的克积极的 eubacteria。我们这里报导奴卡氏菌属 sp 的最新识别的原生质标志 pXT107。107,最小的圆形的原生质标志之一在奴卡氏菌属发现了。pXT107 的完全的核苷酸顺序与 65% G+C 内容由 4335 bp 组成了,并且编码了一复制额外的遗传因子的 palindromic (议员) 和六假想蛋白质。议员,双海滨起源和 pXT107 的单个海滨的起源典型 rolling-circle-replication 原生质标志类似于那些,例如奴卡氏菌属的 pNI100, Rhodococcus 的 pRE8424 和 Streptomyces 的 pIJ101。Escherichia coli 奴卡氏菌属梭原生质标志 pHAQ22,包含 pXT107 的 rep 基因,能在奴卡氏菌属然而并非在 Streptomyces 宣传。
Nocardia, Rhodococcus and Streptornyces, all members of the actinomycetes family, are Gram-positive eubacteria with high G+C content and able to form mycelium. We report here a newly identified plasmid pXT107 of Nocardia sp. 107, one of the smallest circular plasmids found in Nocardia. The complete nucleotide sequence of pXT107 consisted of 4335 bp with 65% G+C content, and encoded one replication extragenic palindromic (Rep) and six hypothetical proteins. The Rep, double-strand origin and single-strand origin of pXT107 resembled those of typical rolling-circle-replication plasmids, such as pNI 100 of Nocardia, pRE8424 of Rhodococcus and plJ 101 of Streptomyces. The Escherichia coli-Nocardia shuttle plasmid pHAQ22, containing the rep gene of pXT107, is able to propagate in Nocardia but not in Streptomyces.