中文摘要：

目的：探讨脂肪酸转运酶/白细胞分化抗原36（fatty acid translocase/CD36,FAT/CD36）在高脂饮食诱导的小鼠脂肪组织炎症中的作用。方法：将6周龄雄性C57BL/6J小鼠分别随机分为普通饮食组和高脂饮食组,喂养14周后,ELISA测定血清游离脂肪酸（FFA）含量,应用荧光实时定量PCR和Western blotting检测脂肪组织中FAT/CD36及炎症/趋化因子（IL-1β、IL-6、TNF-α、MCP-1、MIP-1）mRNA和蛋白的表达,免疫组织化学染色检测脂肪组织巨噬细胞浸润,比较高脂喂养14周的野生型小鼠和CD36基因敲除小鼠的脂肪组织炎症反应情况。结果：与普通饮食组相比,高脂饮食能增强C57BL/6J小鼠脂肪组织的FAT/CD36及炎症/趋化因子的表达,促进巨噬细胞在脂肪组织的浸润。与高脂饮食喂养的野生型小鼠相比,CD36基因敲除小鼠的脂肪组织炎症因子、趋化因子表达明显降低,脂肪组织巨噬细胞浸润减少。结论：高脂饮食通过上调脂肪组织FAT/CD36的表达激活了脂肪组织炎症。

英文摘要：

AIM： To investigate the role of fatty acid translocase/CD36 （FAT/CD36） in adipose tissue in-flammation induced by a high-fat diet.METHODS：C57BL/6J mice were fed with a normal-chow diet （ NCD） or a high-fat diet （ HFD） for 14 weeks.The content of free fatty acid （ FFA） in the serum was measured by ELISA.The expression of CD36, cytokines and chemokines at mRNA and protein levels in the adipose tissues was determined by real-time poly-merase chain reaction and Western blotting.Immunohistochemical staining was used to examine the macrophages infiltration in the adipose tissues.The inflammatory responses in CD36 knockout mice and wild type mice with high-fat diet were ana-lyzed.RESULTS：The levels of FAT/CD36 were higher in HFD group than that in NCD group.HFD feeding enhanced the mRNA and protein expression of IL-1β, IL-6, TNF-α, MCP-1 and MIP-1, as well as promoted macrophage infiltration in the adipose tissues.Interestingly, as fed with HFD, the expression of cytokines/chemokines and macrophage infiltration were significantly reduced in adipose tissues of the CD36 knockout mice, compared with the wild type mice.CONCLU-SION：High-fat diet promotes adipose tissue inflammation in the mice in a FAT/CD36-dependent manner.