中文摘要：

为了实现来源于嗜热栖热菌的海藻糖合酶的高效表达，对E.coli BL21（DE3）/pET24a（＋）-TreS基因工程菌在3 L发酵罐进行了发酵工艺优化，获得的最优发酵条件为：诱导温度32 ℃，当OD600达到50时以0.2 g/（L·h）的速率补加乳糖进行诱导产酶，在该条件下发酵35 h时酶活达到472 U/mL，是优化前的2.3倍；进而采用该条件在30 L发酵罐进行了初步放大研究，酶活达到356 U/mL。由于该海藻糖合酶热稳定性好，因此尝试了高温处理破碎E.coli细胞以释放海藻糖合酶，结果表明，80 ℃处理30 min，海藻糖合酶的得率可达72.3％，具有工业化应用前景。

英文摘要：

In order to achieve efficient production of the trehalose synthase from Thermus thermophilus ,thQ optimization of fermentation conditions was investigated in E.coli BL21 （DE3） harboring the plasmid pET24a（＋）-TreS. The optimized fermentation conditions were as follows：the inducing temperature is 32 ℃ ,the adding rate of lactose is 0.2 g/（L·h） When OD600 reached 50. Under the condition,the enzyme activity reached 472 U/mL at cultivation of 35 h,was 2.3 times than before. In addition,the fermentation was performed in 30 L bioreactor under the condition of preceding narrative,the enzyme activity reached 356 U/mL. Because the thermal stability of trehalose synthase is good, E.coli cell was broken up through high temperature. The results showed that treated it 30 min under 80℃ the yield of trehalose synthase reached 72.3%. It have industrial application prospect.