中文摘要：

目的：研究钙拮抗剂（维拉帕米、地尔硫革和硝苯地平）对大鼠心肌缺血再灌注（I／R）革早期生长反应基因-1（Egr-1）表达的影响。方法：大鼠随机分为假手术组、I／R组、二甲基亚砜组、维拉帕米组、地尔硫革组、硝苯地平组。Westem blot法测定Egr-1蛋白表达水平的变化；RT-PCR法测定Egr-1 mRNA表达水平的变化。结果：与假手术组比，L／R组Egr-1蛋白及Egr-1 mRNA表达显著增高（P〈0．05）；与I／R组比，维拉帕米组、地尔硫革组和硝苯地平组Egr-1蛋白及Egr-1 mRNA表达明显减少（P〈0．05）。结论：维拉帕米、地尔硫革和硝苯地平均可抑制缺血再灌注心肌Egr-1蛋白及Egr-1 mRNA的过度表达。

英文摘要：

Objective： To study the effects of calcium antagonist（verapamil, diltiazem and nifedipine）on early growth response gene-1（Egr-1）expression in rats after myocardial ischemia-reperfusion（I/R）. Methods： Rats were randomly assigned into Sham, I/R, DMSO, vempamil, diltiazem, nifedipine groups. The expression levels of Egr-1 protein and mRNA were examined by Western blot and RT-PCR, respectively. Results： Compared with sham group, Egr-1 protein and mRNA of I/R group overexpressed（ P 〈 0.05）. The expression levels of Egr-1 of vempamil, diltiazem, nifedipine groups significantly decreased, compared with I/R group（ P 〈 0.05）. Conclusion： Verapamil, diltiazem and nifedipine ean inhibit the overexpression of Egr-1 protein and mRNA in rots caused by I/R.