【目的】了解59份美国西北部小麦品种(系)对中国当前条锈菌流行小种的抗性水平,分析其抗病基因的存在情况,为培育中国抗条锈病新品种提供资源和依据。【方法】选用中国小麦条锈菌优势小种条中31号、条中32号和条中33号对大部分具有高温成株抗性的美国59份小麦品种(系)苗期及成株期进行抗性接种鉴定,利用-9当前有效的金生育期抗性基因Yr10,Yr15、成株抗性基因Yr18、高温成株抗性基因Yr39紧密连锁的分子标记对供试材料进行分子检测。【结果】抗性鉴定表明,Expresso、02W50076、ACS52610、WA008012、WA008018等5份材料苗期与成株期对条中31号、条中32号和条中33号混合菌均表现为抗病,属于全生育期抗病品种。33份材料苗期表现感病而成株期表现抗病,属于成株期抗病品种。分子检测发现59份材料均不含有Yr10,含有Yr15,Yr18和厅刀的材料分别为12、33和29份,占20%、56%和50%。【结论】Yr18、肝拶在美国西北部小麦品(系)中的分布较普遍,并且大部分可以抵抗中国当前流行的条锈菌生理小种,可应用这些抗性材料配制中国小麦抗条锈病新品种。
[Objective] In order to clarify resistance levels and existing Yr genes among 59 wheat cultivars (lines) from the Pacific Northwest (PNW) of the United States, to provide resistance resources for genetic improvement of wheat stripe rust resistance in China. [ Method] The 59 PNW wheat cultivars (lines) were infected by 3 mixed races of predominant Chinese stripe rust races CRY31, CRY32 and CRY33 to evaluate their resistance at seedling and adult plant stages, and screened with molecular markers tightly linked to currently effective all-stage resistance genes YrlO, Yr15 and adult plant resistance genes Yr18, Yr39. [Result] Of 59 PNW cultivars (lines), five cultivars (lines) Expresso, 02W50076, ACS52610, WA008012 and WA008018 are all-stage resistance, showing resistance to mixed races of CRY31, CRY32 and CRY33 at both seedling and adult plant stages. 33 cultivars (lines) are adult plant resistance, only showing resistance to stripe rust at adult stage. Based on the molecular screening, none of the 59 PNW cultivars (lines) had the polymorphic bands of lingked markers to YrlO. There are 12, 33 and 29 cultivars (lines) had polymorphic bands of lingked markers to Yrl5, Yr18 and Yr39, making up 20%, 56% and 49% of the 59 PNW cultivars (lines), respectively. [Conclusion ] All these results suggested that Yr18 and Yr39 were widespread among PNW cultivars (cultivars) and can be utilized in Chinese wheat stripe rust resistance breeding.