中文摘要：

利用远红外成像技术筛选得到一株T-DNA插入的干旱敏感突变体，并成功利用TAIL-PCR技术克隆到该突变基因为NCED3,该基因编码植物体内ABA合成的关键限速酶，突变体在干旱胁迫下不能合成ABA而表现出不耐干旱的特征，最后通过PCR及PT-PCR方法验证了TAIL-PCR结果的可靠性。

英文摘要：

By using infrared thermal imaging, we screened a drought sensitive mutant and cloned this mutant gene NCED3 by TAIL-PCR method, which is considered to be the rate-limiting step in abscisic acid （ABA） biosynthesis. The mutant line could not produc ABA under drought stress and showed decreased drought-tolerance phenotype. Suhsequently, PCR and RT-PCR methods were used to confirm the authenticity of TAIL-PCR result.