中文摘要：

目的 构建microR-338-3p（miR-338-3p）过表达载体并探究其对人胃癌SGC-7901细胞系体外增殖的影响.方法 以pcDNATM 6.2-GW/EmGFP-miR真核表达载体为基础,经EcoR Ⅰ和HindⅢ双酶切和纯化后与人工合成miR-338-3p的寡聚核苷酸进行连接,转化至E.Coli Top10后,经过DNA测序、BLAST检测及验证;将构建成功的载体瞬时转染入SGC-7901细胞系中,应用Real-time PCR检测miR-338-3p的表达水平,利用MTT法检测细胞增殖的改变.结果 测序验证成功构建了miR-338-3p的真核表达载体;瞬时转染SGC-7901细胞后miR-338-3p表达水平有显著增加,能够抑制SGC-7901细胞的体外增殖.结论 成功构建了miR-338-3p的真核表达载体,获得了瞬时表达miR-338-3p的人胃癌SGC-7901细胞系,初步证实miR-338-3p过表达可抑制肿瘤细胞的增殖.

英文摘要：

Objective To construct an overexpression vector of microR-338-3p （miR-338-3p） and detect its effect on the proliferation of human gastric cancer cell line SGC-7901.Methods pcDNATM6.2-GW/EmGFP-miR eukaryotic expression vector was cut using restriction site of Hind Ⅲ and EcoR Ⅰ,connected to miR-338-3p oligonucleotide,and identified by sequencing and blast.miR-338-3p overexpression vector was transfected into SGC-7901 cells and the expression level was determined by real-time PCR.The proliferation of SGC-7901 cells was also examined by MTT.Results The overexpression vector of miR-338-3p was identified as real by sequencing.The expression level of miR-338-3p in SGC-7901 cells with expression vectors transfected was increased significantly （P＜0.05）.The proliferation of SGC-7901 cells in vitro was also inhibited.Conclusion The overexpression vector of miR-338-3p was successfully constructed and human gastric cancer cell SGC-7901 instantaneously expressing miR-338-3p was obtained,which preliminarily confirmed that the overexpression of miR-338-3p can inhibit the proliferation of cancer cells.