中文摘要：

目的：探讨降钙素基因相关肽（CGRP）对臭氧（O3）应激后人支气管上皮细胞（HBECs）中E-钙粘素（E-cd）表达的影响及机制。方法：采用RT-PCR检测E-cd mRNA的表达，免疫细胞化学染色法检测E-cd蛋白的表达。结果：CGRP呈剂量依赖性增加正常以及O3应激后HBECs胞膜上E-cd的表达，而对胞浆内E-cd的表达无明显影响；CGRP对HBECs胞膜上E-cd表达的上调作用可分别被H-89（PKA抑制剂）、H-7（PKC抑制剂）及W-7（CaM抑制剂）部分逆转。结论：CGRP可剂量依赖性增加正常和O3应激后HBECs胞膜E-cd的表达，而对胞浆内E-cd的表达无影响。

英文摘要：

Objective： To discuss the effect of calcitonin gene-related peptides （CGRP） on epithelial cadherin （E-cd） expression in human bronchial epithelial cells（HBECs） in vitro. Methods： The effect of CGRP on E-cd protein and mRNA expression in both normal and O3- challenged HBECs were determined by immunocytochemistry and RT-PCR. The signal transducfion pathways of CGRP were observed by using protein kinase C（PKC） inhibitor （H-7）, calmodulin（CaM） inhibitor （W-7） and PKA inhibitor （H-89）. Results： CGRP increased E-cd mRNA and protein expressions of normal and O3-challenged HBECs in a dose-dependent manner. CGRP had no effect on cytoplasm E-cd expression. Pre-treatment with H-89, H-7 and W-7, the up-regulatory effect of CGRP on E-cd expression was partly abolished. Conclusion： CGRP increased in cytomembrane E-cd expression of normal and O3-challenged HBECs in a dose-dependent manner. E-cd expression on HBECs was strengthened by CGRP via PKA, PKC and CaM pathways.